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Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response.

Publication ,  Journal Article
Lotz, MM; Burdsal, CA; Erickson, HP; McClay, DR
Published in: The Journal of cell biology
October 1989

Cell-substratum adhesion strengths have been quantified using fibroblasts and glioma cells binding to two extracellular matrix proteins, fibronectin and tenascin. A centrifugal force-based adhesion assay was used for the adhesive strength measurements, and the corresponding morphology of the adhesions was visualized by interference reflection microscopy. The initial adhesions as measured at 4 degrees C were on the order of 10(-5)dynes/cell and did not involve the cytoskeleton. Adhesion to fibronectin after 15 min at 37 degrees C were more than an order of magnitude stronger; the strengthening response required cytoskeletal involvement. By contrast to the marked strengthening of adhesion to FN, adhesion to TN was unchanged or weakened after 15 min at 37 degrees C. The absolute strength of adhesion achieved varied according to protein and cell type. When a mixed substratum of fibronectin and tenascin was tested, the presence of tenascin was found to reduce the level of the strengthening of cell adhesion normally observed at 37 degrees C on a substratum of fibronectin alone. Parallel analysis of corresponding interference reflection micrographs showed that differences in the area of cell surface within 10-15 nm of the substratum correlated closely with each of the changes in adhesion observed: after incubation for 15 min on fibronectin at 37 degrees C, glioma cells increased their surface area within close contact to the substrate by integral to 125-fold. Cells on tenascin did not increase their surface area of contact. The increased surface area of contact and the inhibitory activity of cytochalasin b suggest that the adhesive "strengthening" in the 15 min after initial binding brings additional adhesion molecules into the adhesive site and couples the actin cytoskeleton to the adhesion complex.

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Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

October 1989

Volume

109

Issue

4 Pt 1

Start / End Page

1795 / 1805

Related Subject Headings

  • Tenascin
  • Neoplasm Proteins
  • Kinetics
  • Humans
  • Glioma
  • Fibronectins
  • Fibroblasts
  • Developmental Biology
  • Cytochalasin B
  • Cell Line
 

Citation

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Lotz, M. M., Burdsal, C. A., Erickson, H. P., & McClay, D. R. (1989). Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response. The Journal of Cell Biology, 109(4 Pt 1), 1795–1805. https://doi.org/10.1083/jcb.109.4.1795
Lotz, M. M., C. A. Burdsal, H. P. Erickson, and D. R. McClay. “Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response.The Journal of Cell Biology 109, no. 4 Pt 1 (October 1989): 1795–1805. https://doi.org/10.1083/jcb.109.4.1795.
Lotz MM, Burdsal CA, Erickson HP, McClay DR. Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response. The Journal of cell biology. 1989 Oct;109(4 Pt 1):1795–805.
Lotz, M. M., et al. “Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response.The Journal of Cell Biology, vol. 109, no. 4 Pt 1, Oct. 1989, pp. 1795–805. Epmc, doi:10.1083/jcb.109.4.1795.
Lotz MM, Burdsal CA, Erickson HP, McClay DR. Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response. The Journal of cell biology. 1989 Oct;109(4 Pt 1):1795–1805.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

October 1989

Volume

109

Issue

4 Pt 1

Start / End Page

1795 / 1805

Related Subject Headings

  • Tenascin
  • Neoplasm Proteins
  • Kinetics
  • Humans
  • Glioma
  • Fibronectins
  • Fibroblasts
  • Developmental Biology
  • Cytochalasin B
  • Cell Line