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Near full-length clones and reference sequences for subtype C isolates of HIV type 1 from three different continents.

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Rodenburg, CM; Li, Y; Trask, SA; Chen, Y; Decker, J; Robertson, DL; Kalish, ML; Shaw, GM; Allen, S; Hahn, BH; Gao, F ...
Published in: AIDS Res Hum Retroviruses
January 20, 2001

Among the major circulating HIV-1 subtypes, subtype C is the most prevalent. To generate full-length subtype C clones and sequences, we selected 13 primary (PBMC-derived) isolates from Zambia, India, Tanzania, South Africa, Brazil, and China, which were identified as subtype C by partial sequence analysis. Near full-length viral genomes were amplified by using a long PCR technique, sequenced in their entirety, and phylogenetically analyzed. Amino acid sequence analysis revealed 10.2, 6.3, and 17.3% diversity in predicted Gag, Pol, and Env protein sequences. Ten of 13 viruses were nonmosaic subtype C genomes, while all three isolates from China represented B/C recombinants. One of them was composed primarily of subtype C sequences with three small subtype B portions in gag, pol, and nef genes. Two others exhibited these same mosaic regions, but contained two additional subtype B portions at the gag/pol overlap and in the accessory gene region, suggesting ongoing B/C recombination in China. All subtype C genomes contained a prematurely truncated second exon of rev, but other previously proposed subtype C signatures, including three potential NF-kappa B-binding sites in the viral promoter-enhancer regions, were found in only a subset of these genomes.

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Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

January 20, 2001

Volume

17

Issue

2

Start / End Page

161 / 168

Location

United States

Related Subject Headings

  • rev Gene Products, Human Immunodeficiency Virus
  • Virology
  • Tanzania
  • South Africa
  • Sequence Alignment
  • Phylogeny
  • Molecular Sequence Data
  • Middle Aged
  • Male
  • India
 

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Rodenburg, C. M., Li, Y., Trask, S. A., Chen, Y., Decker, J., Robertson, D. L., … UNAIDS and NIAID Networks for HIV Isolation and Characterization, . (2001). Near full-length clones and reference sequences for subtype C isolates of HIV type 1 from three different continents. AIDS Res Hum Retroviruses. United States. https://doi.org/10.1089/08892220150217247
Rodenburg, C. M., Y. Li, S. A. Trask, Y. Chen, J. Decker, D. L. Robertson, M. L. Kalish, et al. “Near full-length clones and reference sequences for subtype C isolates of HIV type 1 from three different continents.AIDS Res Hum Retroviruses, January 20, 2001. https://doi.org/10.1089/08892220150217247.
Rodenburg CM, Li Y, Trask SA, Chen Y, Decker J, Robertson DL, et al. Near full-length clones and reference sequences for subtype C isolates of HIV type 1 from three different continents. Vol. 17, AIDS Res Hum Retroviruses. 2001. p. 161–8.
Rodenburg, C. M., et al. “Near full-length clones and reference sequences for subtype C isolates of HIV type 1 from three different continents.AIDS Res Hum Retroviruses, vol. 17, no. 2, 20 Jan. 2001, pp. 161–68. Pubmed, doi:10.1089/08892220150217247.
Rodenburg CM, Li Y, Trask SA, Chen Y, Decker J, Robertson DL, Kalish ML, Shaw GM, Allen S, Hahn BH, Gao F, UNAIDS and NIAID Networks for HIV Isolation and Characterization. Near full-length clones and reference sequences for subtype C isolates of HIV type 1 from three different continents. AIDS Res Hum Retroviruses. 2001. p. 161–168.
Journal cover image

Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

January 20, 2001

Volume

17

Issue

2

Start / End Page

161 / 168

Location

United States

Related Subject Headings

  • rev Gene Products, Human Immunodeficiency Virus
  • Virology
  • Tanzania
  • South Africa
  • Sequence Alignment
  • Phylogeny
  • Molecular Sequence Data
  • Middle Aged
  • Male
  • India