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Cystine and lysine transport in cultured human renal epithelial cells.

Publication ,  Journal Article
States, B; Foreman, J; Lee, J; Harris, D; Segal, S
Published in: Metabolism
April 1987

The transport of the amino acids, cystine and lysine, was studied in epithelial cell lines propagated from human kidney cortex. Cystine uptake data were reproducible in different cell lines and did not vary over several cell passages of an individual cell line. The transport of this disulfide amino acid was sodium-dependent with kinetic analysis showing one apparent Kt system of 0.09 mmol/L and Vmax of 0.054 mmol/L cell water/min. Studies of the kinetics of lysine transport, however, revealed two uptake systems with apparent high and low affinities with Kt of 0.14 mmol/L and 5 mmol/L and Vmax of 0.041 and 0.167 mmol/L cell water/min, respectively. Glutamate appeared to be the most potent inhibitor of cystine uptake by these cultured human renal cells and this interaction was competitive. Although cystine did not inhibit lysine uptake, arginine and ornithine were shown to be major inhibitors, thus providing evidence for the presence of a shared dibasic amino acid transport system.

Duke Scholars

Published In

Metabolism

DOI

ISSN

0026-0495

Publication Date

April 1987

Volume

36

Issue

4

Start / End Page

356 / 362

Location

United States

Related Subject Headings

  • Sodium
  • Ornithine
  • Lysine
  • Kinetics
  • Kidney
  • Humans
  • Epithelium
  • Endocrinology & Metabolism
  • Cystine
  • Biological Transport
 

Citation

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States, B., Foreman, J., Lee, J., Harris, D., & Segal, S. (1987). Cystine and lysine transport in cultured human renal epithelial cells. Metabolism, 36(4), 356–362. https://doi.org/10.1016/0026-0495(87)90207-1
States, B., J. Foreman, J. Lee, D. Harris, and S. Segal. “Cystine and lysine transport in cultured human renal epithelial cells.Metabolism 36, no. 4 (April 1987): 356–62. https://doi.org/10.1016/0026-0495(87)90207-1.
States B, Foreman J, Lee J, Harris D, Segal S. Cystine and lysine transport in cultured human renal epithelial cells. Metabolism. 1987 Apr;36(4):356–62.
States, B., et al. “Cystine and lysine transport in cultured human renal epithelial cells.Metabolism, vol. 36, no. 4, Apr. 1987, pp. 356–62. Pubmed, doi:10.1016/0026-0495(87)90207-1.
States B, Foreman J, Lee J, Harris D, Segal S. Cystine and lysine transport in cultured human renal epithelial cells. Metabolism. 1987 Apr;36(4):356–362.
Journal cover image

Published In

Metabolism

DOI

ISSN

0026-0495

Publication Date

April 1987

Volume

36

Issue

4

Start / End Page

356 / 362

Location

United States

Related Subject Headings

  • Sodium
  • Ornithine
  • Lysine
  • Kinetics
  • Kidney
  • Humans
  • Epithelium
  • Endocrinology & Metabolism
  • Cystine
  • Biological Transport