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In vitro selection and characterization of human immunodeficiency virus type 2 with decreased susceptibility to lopinavir.

Publication ,  Journal Article
Masse, S; Lu, X; Dekhtyar, T; Lu, L; Koev, G; Gao, F; Mo, H; Kempf, D; Bernstein, B; Hanna, GJ; Molla, A
Published in: Antimicrob Agents Chemother
September 2007

Lopinavir (LPV)-ritonavir has demonstrated durable antiviral activity in human immunodeficiency virus type 1 (HIV-1)-infected antiretroviral-naïve and protease inhibitor (PI)-experienced patients. However, information on LPV activity against HIV-2 and the patterns of mutations in HIV-2 in response to selection by LPV is limited. The activity of LPV against three strains of HIV-2 was assessed and compared to activity against a reference HIV-1 strain. LPV demonstrated activity similar to that observed against HIV-1 in two HIV-2 strains (HIV-2(MS) and HIV-2(CBL-23)) tested. On the other hand, approximately 10-fold-reduced susceptibility was observed with the third HIV-2 strain, HIV-2(CDC310319). Passage of HIV-2(MS) with increasing concentrations of LPV selected mutations V47A and D17N in the HIV-2 protease gene. The introduction of both 17N and 47A either individually or together into HIV-2(ROD) molecular infectious clones showed that the single V47A substitution in HIV-2 resulted in a substantial reduction in susceptibility to LPV. In contrast, this mutant retained wild-type susceptibility to other PIs and appeared to be hypersusceptible to atazanavir and saquinavir.

Duke Scholars

Published In

Antimicrob Agents Chemother

DOI

ISSN

0066-4804

Publication Date

September 2007

Volume

51

Issue

9

Start / End Page

3075 / 3080

Location

United States

Related Subject Headings

  • Virus Replication
  • RNA-Directed DNA Polymerase
  • Pyrimidinones
  • Phenotype
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Microbiology
  • Lopinavir
  • Humans
  • HIV-2
 

Citation

APA
Chicago
ICMJE
MLA
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Masse, S., Lu, X., Dekhtyar, T., Lu, L., Koev, G., Gao, F., … Molla, A. (2007). In vitro selection and characterization of human immunodeficiency virus type 2 with decreased susceptibility to lopinavir. Antimicrob Agents Chemother, 51(9), 3075–3080. https://doi.org/10.1128/AAC.00146-07
Masse, Sherie, Xiaozhi Lu, Tatyana Dekhtyar, Liangjun Lu, Gennadiy Koev, Feng Gao, Hongmei Mo, et al. “In vitro selection and characterization of human immunodeficiency virus type 2 with decreased susceptibility to lopinavir.Antimicrob Agents Chemother 51, no. 9 (September 2007): 3075–80. https://doi.org/10.1128/AAC.00146-07.
Masse S, Lu X, Dekhtyar T, Lu L, Koev G, Gao F, et al. In vitro selection and characterization of human immunodeficiency virus type 2 with decreased susceptibility to lopinavir. Antimicrob Agents Chemother. 2007 Sep;51(9):3075–80.
Masse, Sherie, et al. “In vitro selection and characterization of human immunodeficiency virus type 2 with decreased susceptibility to lopinavir.Antimicrob Agents Chemother, vol. 51, no. 9, Sept. 2007, pp. 3075–80. Pubmed, doi:10.1128/AAC.00146-07.
Masse S, Lu X, Dekhtyar T, Lu L, Koev G, Gao F, Mo H, Kempf D, Bernstein B, Hanna GJ, Molla A. In vitro selection and characterization of human immunodeficiency virus type 2 with decreased susceptibility to lopinavir. Antimicrob Agents Chemother. 2007 Sep;51(9):3075–3080.

Published In

Antimicrob Agents Chemother

DOI

ISSN

0066-4804

Publication Date

September 2007

Volume

51

Issue

9

Start / End Page

3075 / 3080

Location

United States

Related Subject Headings

  • Virus Replication
  • RNA-Directed DNA Polymerase
  • Pyrimidinones
  • Phenotype
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Microbiology
  • Lopinavir
  • Humans
  • HIV-2