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Characterization of a thymus-tropic HIV-1 isolate from a rapid progressor: role of the envelope.

Publication ,  Journal Article
Meissner, EG; Duus, KM; Gao, F; Yu, X-F; Su, L
Published in: Virology
October 10, 2004

Loss of T cell homeostasis usually precedes the onset of AIDS. We hypothesized that rapid progressors may be transmitted with HIV-1 that is particularly able to perturb T cell homeostasis. To this end, we have tested two transmitted, syncytium-inducing (SI) viral isolates from a rapid progressor in two thymus models. One of the isolates (R3A) exhibited markedly rapid kinetics of replication and thymocyte depletion. These phenotypes mapped to the envelope, as a recombinant NL4-3 virus encoding the R3A envelope had similar phenotypes, even in the absence of nef. Notably, the viruses with high pathogenic activity in the thymus (R3A and NL4-R3A) did not show enhanced replication or cytopathicity in PHA-stimulated PBMCs. Furthermore, NL4-R3A did not enhance replication of the coinfected NL4-3 virus in the thymus, suggesting an intrinsic advantage of the R3A envelope. The R3A envelope showed higher entry activity in infecting human T cells and in depleting CD4+ thymocytes when expressed in trans. These data suggest that SI viruses with unique envelope functions which can overcome barriers to transmission may hasten disease progression by perturbing T cell homeostasis.

Duke Scholars

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

October 10, 2004

Volume

328

Issue

1

Start / End Page

74 / 88

Location

United States

Related Subject Headings

  • nef Gene Products, Human Immunodeficiency Virus
  • Virus Replication
  • Virology
  • Viral Envelope Proteins
  • Thymus Gland
  • Phenotype
  • Organ Culture Techniques
  • Molecular Sequence Data
  • Mice, SCID
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Meissner, E. G., Duus, K. M., Gao, F., Yu, X.-F., & Su, L. (2004). Characterization of a thymus-tropic HIV-1 isolate from a rapid progressor: role of the envelope. Virology, 328(1), 74–88. https://doi.org/10.1016/j.virol.2004.07.019
Meissner, Eric G., Karen M. Duus, Feng Gao, Xiao-Fang Yu, and Lishan Su. “Characterization of a thymus-tropic HIV-1 isolate from a rapid progressor: role of the envelope.Virology 328, no. 1 (October 10, 2004): 74–88. https://doi.org/10.1016/j.virol.2004.07.019.
Meissner EG, Duus KM, Gao F, Yu X-F, Su L. Characterization of a thymus-tropic HIV-1 isolate from a rapid progressor: role of the envelope. Virology. 2004 Oct 10;328(1):74–88.
Meissner, Eric G., et al. “Characterization of a thymus-tropic HIV-1 isolate from a rapid progressor: role of the envelope.Virology, vol. 328, no. 1, Oct. 2004, pp. 74–88. Pubmed, doi:10.1016/j.virol.2004.07.019.
Meissner EG, Duus KM, Gao F, Yu X-F, Su L. Characterization of a thymus-tropic HIV-1 isolate from a rapid progressor: role of the envelope. Virology. 2004 Oct 10;328(1):74–88.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

October 10, 2004

Volume

328

Issue

1

Start / End Page

74 / 88

Location

United States

Related Subject Headings

  • nef Gene Products, Human Immunodeficiency Virus
  • Virus Replication
  • Virology
  • Viral Envelope Proteins
  • Thymus Gland
  • Phenotype
  • Organ Culture Techniques
  • Molecular Sequence Data
  • Mice, SCID
  • Mice