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Molecular epidemiology of human immunodeficiency virus type 1 transmission in a heterosexual cohort of discordant couples in Zambia.

Publication ,  Journal Article
Trask, SA; Derdeyn, CA; Fideli, U; Chen, Y; Meleth, S; Kasolo, F; Musonda, R; Hunter, E; Gao, F; Allen, S; Hahn, BH
Published in: J Virol
January 2002

Most human immunodeficiency virus type 1 (HIV-1) transmissions in sub-Saharan Africa are believed to occur between married adults who are discordant for their HIV-1 infection status; however, no studies to date have investigated the molecular epidemiology of such transmission events. Here we report the genetic characterization of HIV-1 strains from 149 transmission pairs that were identified prospectively in a cohort of discordant couples in Lusaka, Zambia. Subgenomic gag, gp120, gp41, and/or long terminal repeat regions were amplified by PCR analysis of uncultured blood samples from both partners and sequenced without interim cloning. Pairwise genetic distances were calculated for the regions analyzed and compared to those of subtype-specific reference sequences as well as local controls. Sequence relationships were also examined by phylogenetic tree analysis. By these approaches, epidemiological linkage was established for the majority of transmission pairs. Viruses from 129 of the 149 couples (87%) were very closely related and clustered together in phylogenetic trees in a statistically highly significant manner. In contrast, viruses from 20 of the 149 couples (13%) were only distantly related in two independent genomic regions, thus ruling out transmission between the two partners. The great majority (95%) of transmitted viruses were of subtype C origin, although representatives of subtypes A, D, G, and J were also identified. There was no evidence for extensive transmission networks within the cohort, although two phylogenetic subclusters of viruses infecting two couples each were identified. Taken together, these data indicate that molecular epidemiological analyses of presumed transmission pairs are both feasible and required to determine behavioral, virological, and immunological correlates of heterosexual transmission in sub-Saharan Africa with a high level of accuracy.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 2002

Volume

76

Issue

1

Start / End Page

397 / 405

Location

United States

Related Subject Headings

  • Zambia
  • Virology
  • RNA, Viral
  • Phylogeny
  • Humans
  • Heterosexuality
  • HIV-1
  • HIV Infections
  • Family Relations
  • Cohort Studies
 

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Trask, S. A., Derdeyn, C. A., Fideli, U., Chen, Y., Meleth, S., Kasolo, F., … Hahn, B. H. (2002). Molecular epidemiology of human immunodeficiency virus type 1 transmission in a heterosexual cohort of discordant couples in Zambia. J Virol, 76(1), 397–405. https://doi.org/10.1128/jvi.76.1.397-405.2002
Trask, Stanley A., Cynthia A. Derdeyn, Ulgen Fideli, Yalu Chen, Sreelatha Meleth, Francis Kasolo, Rosemary Musonda, et al. “Molecular epidemiology of human immunodeficiency virus type 1 transmission in a heterosexual cohort of discordant couples in Zambia.J Virol 76, no. 1 (January 2002): 397–405. https://doi.org/10.1128/jvi.76.1.397-405.2002.
Trask SA, Derdeyn CA, Fideli U, Chen Y, Meleth S, Kasolo F, et al. Molecular epidemiology of human immunodeficiency virus type 1 transmission in a heterosexual cohort of discordant couples in Zambia. J Virol. 2002 Jan;76(1):397–405.
Trask, Stanley A., et al. “Molecular epidemiology of human immunodeficiency virus type 1 transmission in a heterosexual cohort of discordant couples in Zambia.J Virol, vol. 76, no. 1, Jan. 2002, pp. 397–405. Pubmed, doi:10.1128/jvi.76.1.397-405.2002.
Trask SA, Derdeyn CA, Fideli U, Chen Y, Meleth S, Kasolo F, Musonda R, Hunter E, Gao F, Allen S, Hahn BH. Molecular epidemiology of human immunodeficiency virus type 1 transmission in a heterosexual cohort of discordant couples in Zambia. J Virol. 2002 Jan;76(1):397–405.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 2002

Volume

76

Issue

1

Start / End Page

397 / 405

Location

United States

Related Subject Headings

  • Zambia
  • Virology
  • RNA, Viral
  • Phylogeny
  • Humans
  • Heterosexuality
  • HIV-1
  • HIV Infections
  • Family Relations
  • Cohort Studies