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Genetically divergent strains of human immunodeficiency virus type 2 use multiple coreceptors for viral entry.

Publication ,  Journal Article
Owen, SM; Ellenberger, D; Rayfield, M; Wiktor, S; Michel, P; Grieco, MH; Gao, F; Hahn, BH; Lal, RB
Published in: J Virol
July 1998

Several members of the seven-transmembrane chemokine receptor family have been shown to serve, with CD4, as coreceptors for entry by human immunodeficiency virus type 1 (HIV-1). While coreceptor usage by HIV-1 primary isolates has been studied by several groups, there is only limited information available concerning coreceptor usage by primary HIV-2 isolates. In this study, we have analyzed coreceptor usage of 15 primary HIV-2 isolates, using lymphocytes from a donor with nonfunctional CCR5 (CCR5 -/-; homozygous 32-bp deletion). Based on the infections of PBMCs, seven of these primary isolates had an absolute requirement for CCR5 expression, whereas the remaining eight exhibited a broader coreceptor usage. All CCR5-requiring isolates were non-syncytium inducing, whereas isolates utilizing multiple coreceptors were syncytium inducing. Blocking experiments using known ligands for chemokine receptors provided indirect evidence for additional coreceptor utilization by primary HIV-2 isolates. Analysis of GHOST4 cell lines expressing various chemokine receptors (CCR1, CCR2b, CCR3, CCR4, CCR5, CXCR4, BONZO, and BOB) further defined specific coreceptor usage of primary HIV-2 isolates. The receptors used included CXCR4, CCR1-5, and the recently described receptors BONZO and BOB. However, the efficiency at which the coreceptors were utilized varied greatly among the various isolates. Analysis of V3 envelope sequences revealed no specific motif that correlated with coreceptor usage. Our data demonstrate that primary HIV-2 isolates are capable of using a broad range of coreceptors for productive infection in vitro. Additionally, our data suggest that expanded coreceptor usage by HIV-2 may correlate with disease progression.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

July 1998

Volume

72

Issue

7

Start / End Page

5425 / 5432

Location

United States

Related Subject Headings

  • Virology
  • Receptors, Virus
  • Receptors, CCR5
  • Phenotype
  • Molecular Sequence Data
  • Humans
  • HIV-2
  • Genetic Variation
  • Amino Acid Sequence
  • 32 Biomedical and clinical sciences
 

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Owen, S. M., Ellenberger, D., Rayfield, M., Wiktor, S., Michel, P., Grieco, M. H., … Lal, R. B. (1998). Genetically divergent strains of human immunodeficiency virus type 2 use multiple coreceptors for viral entry. J Virol, 72(7), 5425–5432. https://doi.org/10.1128/JVI.72.7.5425-5432.1998
Owen, S. M., D. Ellenberger, M. Rayfield, S. Wiktor, P. Michel, M. H. Grieco, F. Gao, B. H. Hahn, and R. B. Lal. “Genetically divergent strains of human immunodeficiency virus type 2 use multiple coreceptors for viral entry.J Virol 72, no. 7 (July 1998): 5425–32. https://doi.org/10.1128/JVI.72.7.5425-5432.1998.
Owen SM, Ellenberger D, Rayfield M, Wiktor S, Michel P, Grieco MH, et al. Genetically divergent strains of human immunodeficiency virus type 2 use multiple coreceptors for viral entry. J Virol. 1998 Jul;72(7):5425–32.
Owen, S. M., et al. “Genetically divergent strains of human immunodeficiency virus type 2 use multiple coreceptors for viral entry.J Virol, vol. 72, no. 7, July 1998, pp. 5425–32. Pubmed, doi:10.1128/JVI.72.7.5425-5432.1998.
Owen SM, Ellenberger D, Rayfield M, Wiktor S, Michel P, Grieco MH, Gao F, Hahn BH, Lal RB. Genetically divergent strains of human immunodeficiency virus type 2 use multiple coreceptors for viral entry. J Virol. 1998 Jul;72(7):5425–5432.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

July 1998

Volume

72

Issue

7

Start / End Page

5425 / 5432

Location

United States

Related Subject Headings

  • Virology
  • Receptors, Virus
  • Receptors, CCR5
  • Phenotype
  • Molecular Sequence Data
  • Humans
  • HIV-2
  • Genetic Variation
  • Amino Acid Sequence
  • 32 Biomedical and clinical sciences