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Overlapping epitopes in human immunodeficiency virus type 1 gp120 presented by HLA A, B, and C molecules: effects of viral variation on cytotoxic T-lymphocyte recognition.

Publication ,  Journal Article
Wilson, CC; Kalams, SA; Wilkes, BM; Ruhl, DJ; Gao, F; Hahn, BH; Hanson, IC; Luzuriaga, K; Wolinsky, S; Koup, R; Buchbinder, SP; Johnson, RP ...
Published in: J Virol
February 1997

Human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes (CTL) are thought to exert immunologic selection pressure in infected persons, yet few data regarding the effects of this constraint on viral sequence variation in vivo, particularly in the highly variable Env protein, are available. In this study, CD8+ HIV type 1 (HIV-1) envelope-specific CTL clones specific for gp120 were isolated from peripheral blood mononuclear cells of four HIV-infected individuals, all of which recognized the same 25-amino-acid (aa) peptide (aa 371 to 395), which is partially contained in the CD4-binding domain of HIV-1 gp120. Fine mapping studies revealed that two of the clones optimally recognized the 9-aa sequence 375 to 383 (SFNCGGEFF), while the two other clones optimally recognized the epitope contained in the overlapping 9-aa sequence 376 to 384 (FNCGGEFFY). Lysis of target cells by the two clones recognizing aa 375 to 383 was restricted by HLA B15 and Cw4, respectively, whereas both clones recognizing aa 376 to 384 were restricted by HLA A29. Sequence variation, relative to the IIIB strain sequence used to identify CTL clones, was observed in autologous viruses in the epitope-containing region in all four subjects. However, poorly recognized autologous sequence variants were predominantly seen for the A29-restricted clones, whereas the clones specific for SFNCGGEFF continued to recognize the predominant autologous sequences. These results suggest that the HLA profile of an individual may not only be important in determining the specificity of CTL recognition but may also affect the ability to recognize virus variants and suppress escape from CTL recognition. These results also identify overlapping viral CTL epitopes which can be presented by HLA A, B, and C molecules.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

February 1997

Volume

71

Issue

2

Start / End Page

1256 / 1264

Location

United States

Related Subject Headings

  • Virology
  • T-Lymphocytes, Cytotoxic
  • Sequence Analysis
  • Humans
  • HLA-C Antigens
  • HLA-B Antigens
  • HLA-A Antigens
  • HIV Envelope Protein gp120
  • Epitopes
  • Epitope Mapping
 

Citation

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Chicago
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MLA
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Wilson, C. C., Kalams, S. A., Wilkes, B. M., Ruhl, D. J., Gao, F., Hahn, B. H., … Walker, B. D. (1997). Overlapping epitopes in human immunodeficiency virus type 1 gp120 presented by HLA A, B, and C molecules: effects of viral variation on cytotoxic T-lymphocyte recognition. J Virol, 71(2), 1256–1264. https://doi.org/10.1128/JVI.71.2.1256-1264.1997
Wilson, C. C., S. A. Kalams, B. M. Wilkes, D. J. Ruhl, F. Gao, B. H. Hahn, I. C. Hanson, et al. “Overlapping epitopes in human immunodeficiency virus type 1 gp120 presented by HLA A, B, and C molecules: effects of viral variation on cytotoxic T-lymphocyte recognition.J Virol 71, no. 2 (February 1997): 1256–64. https://doi.org/10.1128/JVI.71.2.1256-1264.1997.
Wilson, C. C., et al. “Overlapping epitopes in human immunodeficiency virus type 1 gp120 presented by HLA A, B, and C molecules: effects of viral variation on cytotoxic T-lymphocyte recognition.J Virol, vol. 71, no. 2, Feb. 1997, pp. 1256–64. Pubmed, doi:10.1128/JVI.71.2.1256-1264.1997.
Wilson CC, Kalams SA, Wilkes BM, Ruhl DJ, Gao F, Hahn BH, Hanson IC, Luzuriaga K, Wolinsky S, Koup R, Buchbinder SP, Johnson RP, Walker BD. Overlapping epitopes in human immunodeficiency virus type 1 gp120 presented by HLA A, B, and C molecules: effects of viral variation on cytotoxic T-lymphocyte recognition. J Virol. 1997 Feb;71(2):1256–1264.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

February 1997

Volume

71

Issue

2

Start / End Page

1256 / 1264

Location

United States

Related Subject Headings

  • Virology
  • T-Lymphocytes, Cytotoxic
  • Sequence Analysis
  • Humans
  • HLA-C Antigens
  • HLA-B Antigens
  • HLA-A Antigens
  • HIV Envelope Protein gp120
  • Epitopes
  • Epitope Mapping