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Genetic variation of HIV type 1 in four World Health Organization-sponsored vaccine evaluation sites: generation of functional envelope (glycoprotein 160) clones representative of sequence subtypes A, B, C, and E. WHO Network for HIV Isolation and Characterization.

Publication ,  Journal Article
Gao, F; Yue, L; Craig, S; Thornton, CL; Robertson, DL; McCutchan, FE; Bradac, JA; Sharp, PM; Hahn, BH
Published in: AIDS Res Hum Retroviruses
November 1994

As part of the WHO Network for HIV Isolation and Characterization, we PCR amplified, cloned, and sequenced gp120 and gp160 genes from 12 HIV-1 isolates collected in four WHO-sponsored vaccine evaluation sites (Brazil, Rwanda, Thailand, Uganda). Envelope clones were derived from PBMC-grown isolates obtained from asymptomatic individuals within 2 years of seroconversion. Analysis of their deduced amino acid sequences identified all but one to contain an uninterrupted open reading frame. Transient expression and biological characterization of selected gp160 constructs identified six clones to encode full length and functional envelope glycoproteins. Phylogenetic analysis of their nucleotide sequences revealed that they represent HIV-1 subtypes A, B, C, and E. Since current knowledge of HIV-1 envelope immunobiology is almost exclusively derived from subtype B viruses, these reagents should facilitate future envelope structure, function and antigenicity studies on a broader spectrum of viruses. This should assist in the design and evaluation of effective vaccines against HIV-1.

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Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

November 1994

Volume

10

Issue

11

Start / End Page

1359 / 1368

Location

United States

Related Subject Headings

  • World Health Organization
  • Virology
  • Uganda
  • Thailand
  • Sequence Homology, Amino Acid
  • Rwanda
  • Protein Precursors
  • Polymerase Chain Reaction
  • Phylogeny
  • Molecular Sequence Data
 

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Gao, F., Yue, L., Craig, S., Thornton, C. L., Robertson, D. L., McCutchan, F. E., … Hahn, B. H. (1994). Genetic variation of HIV type 1 in four World Health Organization-sponsored vaccine evaluation sites: generation of functional envelope (glycoprotein 160) clones representative of sequence subtypes A, B, C, and E. WHO Network for HIV Isolation and Characterization. AIDS Res Hum Retroviruses, 10(11), 1359–1368. https://doi.org/10.1089/aid.1994.10.1359
Gao, F., L. Yue, S. Craig, C. L. Thornton, D. L. Robertson, F. E. McCutchan, J. A. Bradac, P. M. Sharp, and B. H. Hahn. “Genetic variation of HIV type 1 in four World Health Organization-sponsored vaccine evaluation sites: generation of functional envelope (glycoprotein 160) clones representative of sequence subtypes A, B, C, and E. WHO Network for HIV Isolation and Characterization.AIDS Res Hum Retroviruses 10, no. 11 (November 1994): 1359–68. https://doi.org/10.1089/aid.1994.10.1359.
Journal cover image

Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

November 1994

Volume

10

Issue

11

Start / End Page

1359 / 1368

Location

United States

Related Subject Headings

  • World Health Organization
  • Virology
  • Uganda
  • Thailand
  • Sequence Homology, Amino Acid
  • Rwanda
  • Protein Precursors
  • Polymerase Chain Reaction
  • Phylogeny
  • Molecular Sequence Data