Scholars@Duke publication: Molecular cloning and sequencing of zeta-crystallin/quinone reductase cDNA from human liver.

Molecular cloning and sequencing of zeta-crystallin/quinone reductase cDNA from human liver.

Publication , Journal Article

Gonzalez, P; Rao, PV; Zigler, JS

Published in: Biochem Biophys Res Commun

March 31, 1993

Zeta-crystallin is an enzyme-crystallin highly expressed in the lens of some hystricomorph rodents and camels. It has been shown to have a novel NADPH: quinone oxidoreductase activity and is present at enzymatic levels in a variety of tissues from various mammals. We report here the cDNA cloning of zeta-crystallin from a human liver library. One clone with the complete open reading frame was obtained. Ten nucleotides of the 5' and 796 of the 3' nontranslated regions are present in the clone including two possible polyadenylation signals. The deduced amino acid sequence is 328 residues long with a calculated molecular mass of 34910 daltons and isoelectric point of 8.73. It shows 84% identity with the guinea pig protein.

Duke Scholars

Author Ponugoti Vasantha Rao Ophthalmology, Glaucoma

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Published In

Biochem Biophys Res Commun

DOI

10.1006/bbrc.1993.1302

ISSN

0006-291X

Publication Date

March 31, 1993

Volume

191

Issue

Start / End Page

902 / 907

Location

United States

Related Subject Headings

Sequence Alignment
Restriction Mapping
NAD(P)H Dehydrogenase (Quinone)
Molecular Sequence Data
Liver
Humans
DNA
Crystallins
Cricetinae
Cloning, Molecular

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Gonzalez, P., Rao, P. V., & Zigler, J. S. (1993). Molecular cloning and sequencing of zeta-crystallin/quinone reductase cDNA from human liver. Biochem Biophys Res Commun, 191(3), 902–907. https://doi.org/10.1006/bbrc.1993.1302

Gonzalez, P., P. V. Rao, and J. S. Zigler. “Molecular cloning and sequencing of zeta-crystallin/quinone reductase cDNA from human liver.” Biochem Biophys Res Commun 191, no. 3 (March 31, 1993): 902–7. https://doi.org/10.1006/bbrc.1993.1302.

Gonzalez P, Rao PV, Zigler JS. Molecular cloning and sequencing of zeta-crystallin/quinone reductase cDNA from human liver. Biochem Biophys Res Commun. 1993 Mar 31;191(3):902–7.

Gonzalez, P., et al. “Molecular cloning and sequencing of zeta-crystallin/quinone reductase cDNA from human liver.” Biochem Biophys Res Commun, vol. 191, no. 3, Mar. 1993, pp. 902–07. Pubmed, doi:10.1006/bbrc.1993.1302.

Gonzalez P, Rao PV, Zigler JS. Molecular cloning and sequencing of zeta-crystallin/quinone reductase cDNA from human liver. Biochem Biophys Res Commun. 1993 Mar 31;191(3):902–907.