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Optical measurements of transmembrane potential changes during electric field stimulation of ventricular cells.

Publication ,  Journal Article
Knisley, SB; Blitchington, TF; Hill, BC; Grant, AO; Smith, WM; Pilkington, TC; Ideker, RE
Published in: Circ Res
February 1993

We evaluated transmembrane potential changes at the ends of isolated rabbit ventricular myocytes during defibrillation-strength shocks given in the cellular refractory period. The myocytes were stimulated (S1 pulse) to produce an action potential. Then a constant-field shock (S2 pulse) with an electric field of 20 or 40 V/cm was given at an S1-S2 interval of 50 msec. The cells were stained with potentiometric dye (di-4-ANEPPS), and the cell end facing the S2 anode or cathode was illuminated with a laser while the fluorescence was recorded. During S2, the cell end facing the S2 cathode became more positive intracellularly, whereas the cell end facing the S2 anode became more negative intracellularly. The S2-induced transmembrane potential change at the cell end (delta Vm) was determined relative to the amplitude of the S1-induced action potential (APA) in each recording (i.e., delta Vm/APA). In Tyrode's solution containing 4.5 mM potassium, delta Vm/APA for 40-V/cm S2 was 1.36 +/- 0.34 at the cell end facing the S2 cathode and -1.65 +/- 0.61 at the cell end facing the S2 anode (n = 9). For the 20-V/cm S2, delta Vm/APA was 0.61 +/- 0.33 at the cell end facing the S2 cathode and -0.71 +/- 0.33 at the cell end facing the S2 anode (n = 6). The delta Vm/APA was not significantly influenced by 20 mM diacetyl monoxime. These results indicate that large delta Vm values occurred at the ends of the cells during S2. The calculated values of delta Vm, assuming a nominal APA of 130 mV, were 177 and -214 mV for the 40-V/cm S2 and 79 and -93 mV for the 20-V/cm S2. The delta Vm was correlated with cell size (r > or = 0.95) and agreed with values predicted by the S2 electric field strength multiplied by half of the cell length to within 27%. When the potassium concentration was increased to 20 mM, delta Vm/APA for 40 V/cm S2 increased 85% and 67% at the cell ends facing the S2 cathode and anode, respectively (n = 9, p < 0.005 versus 4.5 mM potassium), consistent with reduced APA. Thus, with normal or elevated extracellular potassium, transmembrane potential changes at the ends of cells during defibrillation-type stimulation are large enough to produce activation or recovery of voltage-dependent ion channels and may produce the effects responsible for defibrillation.

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Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

February 1993

Volume

72

Issue

2

Start / End Page

255 / 270

Location

United States

Related Subject Headings

  • Ventricular Function
  • Transillumination
  • Rabbits
  • Potassium
  • Membrane Potentials
  • In Vitro Techniques
  • Heart Ventricles
  • Fluorescent Dyes
  • Electric Stimulation
  • Electric Countershock
 

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Knisley, S. B., Blitchington, T. F., Hill, B. C., Grant, A. O., Smith, W. M., Pilkington, T. C., & Ideker, R. E. (1993). Optical measurements of transmembrane potential changes during electric field stimulation of ventricular cells. Circ Res, 72(2), 255–270. https://doi.org/10.1161/01.res.72.2.255
Knisley, S. B., T. F. Blitchington, B. C. Hill, A. O. Grant, W. M. Smith, T. C. Pilkington, and R. E. Ideker. “Optical measurements of transmembrane potential changes during electric field stimulation of ventricular cells.Circ Res 72, no. 2 (February 1993): 255–70. https://doi.org/10.1161/01.res.72.2.255.
Knisley SB, Blitchington TF, Hill BC, Grant AO, Smith WM, Pilkington TC, et al. Optical measurements of transmembrane potential changes during electric field stimulation of ventricular cells. Circ Res. 1993 Feb;72(2):255–70.
Knisley, S. B., et al. “Optical measurements of transmembrane potential changes during electric field stimulation of ventricular cells.Circ Res, vol. 72, no. 2, Feb. 1993, pp. 255–70. Pubmed, doi:10.1161/01.res.72.2.255.
Knisley SB, Blitchington TF, Hill BC, Grant AO, Smith WM, Pilkington TC, Ideker RE. Optical measurements of transmembrane potential changes during electric field stimulation of ventricular cells. Circ Res. 1993 Feb;72(2):255–270.

Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

February 1993

Volume

72

Issue

2

Start / End Page

255 / 270

Location

United States

Related Subject Headings

  • Ventricular Function
  • Transillumination
  • Rabbits
  • Potassium
  • Membrane Potentials
  • In Vitro Techniques
  • Heart Ventricles
  • Fluorescent Dyes
  • Electric Stimulation
  • Electric Countershock