Frequent Imprecise Excision among Reversions of a P Element-Caused Lethal Mutation in Drosophila.
RpII215(D) (50) (= D50) is a lethal mutation caused by the insertion of a 1.3-kb P element 5' to sequences encoding the largest (215 kilodaltons) subunit of Drosophila RNA polymerase II. In dysgenic males D50 reverted to nonlethality at frequencies ranging from 2.6 to 6.5%. These reversions resulted from loss of P element sequences. Genetic tests of function and restriction enzyme analysis of revertant DNAs revealed that 35% or more of the reversion events were imprecise excisions. Two meiotic mutations that perturb excision repair and postreplication repair (mei-9(a) and mei-41(D5), respectively) had no influence on reversion frequency but may have increased the proportion of imprecise excisions. We suggest that these excisions are by-products of, rather than intermediates in, the transposition process.
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- Developmental Biology
- 3105 Genetics
- 3101 Biochemistry and cell biology
- 0604 Genetics
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Developmental Biology
- 3105 Genetics
- 3101 Biochemistry and cell biology
- 0604 Genetics