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[Studies on MDM2 oncogene expression and its effect on pancreatic carcinoma cells].

Publication ,  Journal Article
Guo, H; Liu, T; Gao, J
Published in: Zhonghua Bing Li Xue Za Zhi
August 1996

In order to study the interrelation and interaction between MDM2 oncogene and wild type p53 in human pancreatic cancer, we studied the expression and amplification of MDM2 oncogene and its antagonistic effect on wild type p53 by use of gene recombination, gene transduction and molecular hybridization techniques. The results showed that MDM2 oncogene could be detected in all 5 pancreatic cell lines, but MDM2 mRNA expression varied in the different cell lines. The recombinant vector pCMV-MDM2 was transduced into PC-2/s-wtp53 cell line (a transformed PC-2 pancreatic carcinoma cell line containing wild type p53 gene). The resultant cell line, PC-2/s-wtp53/pCMV-MDM2 showed rapid cell growth, a rate similar to that of the parent cell line PC-2. Our results verify the fact that MDM2 gene can abrogate the cell growth arrest mediated by wild type p53 and the antagonistic function of wild type p53.

Duke Scholars

Published In

Zhonghua Bing Li Xue Za Zhi

ISSN

0529-5807

Publication Date

August 1996

Volume

25

Issue

4

Start / End Page

232 / 235

Location

China

Related Subject Headings

  • Tumor Cells, Cultured
  • Transduction, Genetic
  • RNA, Messenger
  • Proto-Oncogene Proteins c-mdm2
  • Proto-Oncogene Proteins
  • Pathology
  • Pancreatic Neoplasms
  • Nuclear Proteins
  • Mice, Nude
  • Mice, Inbred BALB C
 

Citation

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MLA
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Guo, H., Liu, T., & Gao, J. (1996). [Studies on MDM2 oncogene expression and its effect on pancreatic carcinoma cells]. Zhonghua Bing Li Xue Za Zhi, 25(4), 232–235.
Guo, H., T. Liu, and J. Gao. “[Studies on MDM2 oncogene expression and its effect on pancreatic carcinoma cells].Zhonghua Bing Li Xue Za Zhi 25, no. 4 (August 1996): 232–35.
Guo H, Liu T, Gao J. [Studies on MDM2 oncogene expression and its effect on pancreatic carcinoma cells]. Zhonghua Bing Li Xue Za Zhi. 1996 Aug;25(4):232–5.
Guo, H., et al. “[Studies on MDM2 oncogene expression and its effect on pancreatic carcinoma cells].Zhonghua Bing Li Xue Za Zhi, vol. 25, no. 4, Aug. 1996, pp. 232–35.
Guo H, Liu T, Gao J. [Studies on MDM2 oncogene expression and its effect on pancreatic carcinoma cells]. Zhonghua Bing Li Xue Za Zhi. 1996 Aug;25(4):232–235.

Published In

Zhonghua Bing Li Xue Za Zhi

ISSN

0529-5807

Publication Date

August 1996

Volume

25

Issue

4

Start / End Page

232 / 235

Location

China

Related Subject Headings

  • Tumor Cells, Cultured
  • Transduction, Genetic
  • RNA, Messenger
  • Proto-Oncogene Proteins c-mdm2
  • Proto-Oncogene Proteins
  • Pathology
  • Pancreatic Neoplasms
  • Nuclear Proteins
  • Mice, Nude
  • Mice, Inbred BALB C