Isolation of renal brush borders.
Publication
, Journal Article
Morré, DJ; Hammond, T
Published in: Curr Protoc Cell Biol
March 2007
Methods are described to isolate intact brush borders and brush border membranes from renal cell homogenates. A rapid method yields sealed vesicles that reconstitute renal brush border transport. In one variation of this protocol, 10 to 20 mM CaCl2 or MgCl2 is added to aggregate non-brush border structures for subsequent removal by centrifugation. For analytical studies, guidance is provided for subsequent purification steps including preparative free-flow and aqueous two-phase partition. Marker enzymes and morphological parameters are included for assessment of yield and fraction purity.
Duke Scholars
Published In
Curr Protoc Cell Biol
DOI
EISSN
1934-2616
Publication Date
March 2007
Volume
Chapter 3
Start / End Page
3.26.1 / 3.26.14
Location
United States
Related Subject Headings
- Solubility
- Rats, Sprague-Dawley
- Rats
- Rabbits
- Microvilli
- Microscopy, Electron
- Male
- Magnesium Chloride
- Kidney Cortex
- Endosomes
Citation
APA
Chicago
ICMJE
MLA
NLM
Morré, D. J., & Hammond, T. (2007). Isolation of renal brush borders. Curr Protoc Cell Biol, Chapter 3, 3.26.1-3.26.14. https://doi.org/10.1002/0471143030.cb0326s34
Morré, D James, and Timothy Hammond. “Isolation of renal brush borders.” Curr Protoc Cell Biol Chapter 3 (March 2007): 3.26.1-3.26.14. https://doi.org/10.1002/0471143030.cb0326s34.
Morré DJ, Hammond T. Isolation of renal brush borders. Curr Protoc Cell Biol. 2007 Mar;Chapter 3:3.26.1-3.26.14.
Morré, D. James, and Timothy Hammond. “Isolation of renal brush borders.” Curr Protoc Cell Biol, vol. Chapter 3, Mar. 2007, pp. 3.26.1-3.26.14. Pubmed, doi:10.1002/0471143030.cb0326s34.
Morré DJ, Hammond T. Isolation of renal brush borders. Curr Protoc Cell Biol. 2007 Mar;Chapter 3:3.26.1-3.26.14.
Published In
Curr Protoc Cell Biol
DOI
EISSN
1934-2616
Publication Date
March 2007
Volume
Chapter 3
Start / End Page
3.26.1 / 3.26.14
Location
United States
Related Subject Headings
- Solubility
- Rats, Sprague-Dawley
- Rats
- Rabbits
- Microvilli
- Microscopy, Electron
- Male
- Magnesium Chloride
- Kidney Cortex
- Endosomes