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Neurokinin-1 (NK-1) receptor is required in antigen-induced cystitis.

Publication ,  Journal Article
Saban, R; Saban, MR; Nguyen, NB; Lu, B; Gerard, C; Gerard, NP; Hammond, TG
Published in: Am J Pathol
March 2000

Interstitial cystitis (IC) is a debilitating disease that has been adversely affecting the quality of women's lives for many years. The trigger in IC is not entirely known, and a role for the sensory nerves in its pathogenesis has been suggested. In addition to inflammation, increased mast cell numbers in the detrusor muscle have been reported in a subset of IC patients. Experimentally, several lines of evidence support a central role for substance P and neurokinin-1 (NK-1) receptors in cystitis. The availability of mice genetically deficient in neurokinin-1 receptor (NK-1R(-/-)) allows us to directly evaluate the importance of substance P in cystitis. An unexpected finding of this investigation is that NK-1R(-/-) mice present increased numbers of mast cells in the bladder when compared with wild-type control mice. Despite the increase in mast cell numbers, no concomitant inflammation was observed. In addition, bladder instillation of wild-type mice with a sensitizing antigen induces activation of mast cells and an acute inflammatory response characterized by plasma extravasation, edema, and migration of neutrophils. Antigen-sensitized NK-1R(-/-) mice also exhibit bladder mast cell degranulation in response to antigen challenge. However, NK-1R(-/-) mice are protected from inflammation, failing to present bladder inflammatory cell infiltrate or edema in response to antigen challenge. This work presents the first evidence of participation of NK-1 receptors in cystitis and a mandatory participation of these receptors on the chain of events linking mast cell degranulation and inflammation.

Duke Scholars

Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

March 2000

Volume

156

Issue

3

Start / End Page

775 / 780

Location

United States

Related Subject Headings

  • Urinary Bladder
  • Substance P
  • Receptors, Neurokinin-1
  • Pathology
  • Ovalbumin
  • Mice, Knockout
  • Mice
  • Mast Cells
  • Immunization
  • Hypersensitivity, Immediate
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Saban, R., Saban, M. R., Nguyen, N. B., Lu, B., Gerard, C., Gerard, N. P., & Hammond, T. G. (2000). Neurokinin-1 (NK-1) receptor is required in antigen-induced cystitis. Am J Pathol, 156(3), 775–780. https://doi.org/10.1016/S0002-9440(10)64944-9
Saban, R., M. R. Saban, N. B. Nguyen, B. Lu, C. Gerard, N. P. Gerard, and T. G. Hammond. “Neurokinin-1 (NK-1) receptor is required in antigen-induced cystitis.Am J Pathol 156, no. 3 (March 2000): 775–80. https://doi.org/10.1016/S0002-9440(10)64944-9.
Saban R, Saban MR, Nguyen NB, Lu B, Gerard C, Gerard NP, et al. Neurokinin-1 (NK-1) receptor is required in antigen-induced cystitis. Am J Pathol. 2000 Mar;156(3):775–80.
Saban, R., et al. “Neurokinin-1 (NK-1) receptor is required in antigen-induced cystitis.Am J Pathol, vol. 156, no. 3, Mar. 2000, pp. 775–80. Pubmed, doi:10.1016/S0002-9440(10)64944-9.
Saban R, Saban MR, Nguyen NB, Lu B, Gerard C, Gerard NP, Hammond TG. Neurokinin-1 (NK-1) receptor is required in antigen-induced cystitis. Am J Pathol. 2000 Mar;156(3):775–780.
Journal cover image

Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

March 2000

Volume

156

Issue

3

Start / End Page

775 / 780

Location

United States

Related Subject Headings

  • Urinary Bladder
  • Substance P
  • Receptors, Neurokinin-1
  • Pathology
  • Ovalbumin
  • Mice, Knockout
  • Mice
  • Mast Cells
  • Immunization
  • Hypersensitivity, Immediate