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Identification of rat yolk sac target protein of teratogenic antibodies, gp280, as intrinsic factor-cobalamin receptor.

Publication ,  Journal Article
Seetharam, B; Christensen, EI; Moestrup, SK; Hammond, TG; Verroust, PJ
Published in: J Clin Invest
May 15, 1997

Previous studies in the rat have shown that antibodies to gp280, a protein > 200 kD and closely associated with the early endocytic system can induce fetal malformations. Although gp280 is thought to act as a receptor, its ligand(s) is not known. In the current study, we report that purified gp280 from rat kidney, like the intrinsic factor-Cobalamin receptor (IFCR), binds to the intrinsic factor-cobalamin (IFCbl) complex with an association constant of 0.3 x 10(9) M-1 and mediates its internalization. Furthermore, antibodies raised to purified gp280 and IFCR inhibited the binding of IF-[57Co]Cbl complex to intestinal, renal, and yolk sac apical membranes and revealed a single identically sized protein on immunoblotting of the renal membranes. Both antibodies precipitated a single radiolabeled protein > 200 kD from cellular extract from [35S]methionine-labeled yolk sac epithelial cells, and antibody to gp280 inhibited the uptake and internalization of 125IF-Cbl. Immunoelectron microscopy using the two antibodies revealed that in the kidney, both proteins were colocalized. These observations suggest that IF-Cbl complex is a ligand for gp280 and that gp280 and IFCR are identical proteins.

Duke Scholars

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

May 15, 1997

Volume

99

Issue

10

Start / End Page

2317 / 2322

Location

United States

Related Subject Headings

  • Yolk Sac
  • Teratogens
  • Receptors, Cell Surface
  • Rats
  • Molecular Weight
  • Microvilli
  • Kinetics
  • Kidney
  • Intestinal Mucosa
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Seetharam, B., Christensen, E. I., Moestrup, S. K., Hammond, T. G., & Verroust, P. J. (1997). Identification of rat yolk sac target protein of teratogenic antibodies, gp280, as intrinsic factor-cobalamin receptor. J Clin Invest, 99(10), 2317–2322. https://doi.org/10.1172/JCI119411
Seetharam, B., E. I. Christensen, S. K. Moestrup, T. G. Hammond, and P. J. Verroust. “Identification of rat yolk sac target protein of teratogenic antibodies, gp280, as intrinsic factor-cobalamin receptor.J Clin Invest 99, no. 10 (May 15, 1997): 2317–22. https://doi.org/10.1172/JCI119411.
Seetharam B, Christensen EI, Moestrup SK, Hammond TG, Verroust PJ. Identification of rat yolk sac target protein of teratogenic antibodies, gp280, as intrinsic factor-cobalamin receptor. J Clin Invest. 1997 May 15;99(10):2317–22.
Seetharam, B., et al. “Identification of rat yolk sac target protein of teratogenic antibodies, gp280, as intrinsic factor-cobalamin receptor.J Clin Invest, vol. 99, no. 10, May 1997, pp. 2317–22. Pubmed, doi:10.1172/JCI119411.
Seetharam B, Christensen EI, Moestrup SK, Hammond TG, Verroust PJ. Identification of rat yolk sac target protein of teratogenic antibodies, gp280, as intrinsic factor-cobalamin receptor. J Clin Invest. 1997 May 15;99(10):2317–2322.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

May 15, 1997

Volume

99

Issue

10

Start / End Page

2317 / 2322

Location

United States

Related Subject Headings

  • Yolk Sac
  • Teratogens
  • Receptors, Cell Surface
  • Rats
  • Molecular Weight
  • Microvilli
  • Kinetics
  • Kidney
  • Intestinal Mucosa
  • Immunology