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Segmental distribution of the endocytosis receptor gp330 in renal proximal tubules.

Publication ,  Journal Article
Christensen, EI; Nielsen, S; Moestrup, SK; Borre, C; Maunsbach, AB; de Heer, E; Ronco, P; Hammond, TG; Verroust, P
Published in: Eur J Cell Biol
April 1995

The subcellular distribution and segmental variations in location of gp330, a scavenger receptor for filtered proteins in renal proximal tubules, was analyzed. Kidney tissue from rats (4 different strains), rabbits and humans were analyzed by light- and electron microscope immunocytochemistry, using cryosections or Lowicryl sections from cryosubstituted tissue. Gp330 was located mainly in apical coated pits, small and large endocytic vacuoles and in dense apical tubules in the proximal tubule cells. The labeling density was markedly higher in segments 1 and 2 as compared to segment 3 of the proximal tubule. In addition to the location in the early part of the endocytic pathway, gp330 was also present in lysosomes, especially in segments 1 and 2. The lysosomal labeling was not restricted to the membrane, but was also seen in the matrix. Localization of gp330 in lysosomes was confirmed on sections from purified lysosomal fractions from rat renal cortex. The brush border localization of gp330 in proximal tubules exhibited a characteristic segmental variation. In the initial part of segment 1, there was virtually no brush border labeling. In the remaining part of segment 1 and in segment 2, there was a distinct but sometimes patchy labeling of the brush border. In segment 3, groups of microvilli of approximately 10 as seen in sections were intensively labeled from bottom to tip and there were often more than one of these groups on a single cell, the remaining microvilli were unlabeled. No differences in the cellular and subcellular localization of gp330 were observed between species or rat strains. In conclusion, the present study demonstrates that in addition to its location in the early endocytic and recycling pathway, gp330 is also present in microvilli and the protein and degradation products thereof is present in lysosomes, consistent with its role as a protein scavenger receptor.

Duke Scholars

Published In

Eur J Cell Biol

ISSN

0171-9335

Publication Date

April 1995

Volume

66

Issue

4

Start / End Page

349 / 364

Location

Germany

Related Subject Headings

  • Receptors, LDL
  • Rats, Wistar
  • Rats, Sprague-Dawley
  • Rats, Brattleboro
  • Rats
  • Rabbits
  • Microvilli
  • Microscopy, Electron
  • Membrane Glycoproteins
  • Male
 

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Christensen, E. I., Nielsen, S., Moestrup, S. K., Borre, C., Maunsbach, A. B., de Heer, E., … Verroust, P. (1995). Segmental distribution of the endocytosis receptor gp330 in renal proximal tubules. Eur J Cell Biol, 66(4), 349–364.
Christensen, E. I., S. Nielsen, S. K. Moestrup, C. Borre, A. B. Maunsbach, E. de Heer, P. Ronco, T. G. Hammond, and P. Verroust. “Segmental distribution of the endocytosis receptor gp330 in renal proximal tubules.Eur J Cell Biol 66, no. 4 (April 1995): 349–64.
Christensen EI, Nielsen S, Moestrup SK, Borre C, Maunsbach AB, de Heer E, et al. Segmental distribution of the endocytosis receptor gp330 in renal proximal tubules. Eur J Cell Biol. 1995 Apr;66(4):349–64.
Christensen, E. I., et al. “Segmental distribution of the endocytosis receptor gp330 in renal proximal tubules.Eur J Cell Biol, vol. 66, no. 4, Apr. 1995, pp. 349–64.
Christensen EI, Nielsen S, Moestrup SK, Borre C, Maunsbach AB, de Heer E, Ronco P, Hammond TG, Verroust P. Segmental distribution of the endocytosis receptor gp330 in renal proximal tubules. Eur J Cell Biol. 1995 Apr;66(4):349–364.
Journal cover image

Published In

Eur J Cell Biol

ISSN

0171-9335

Publication Date

April 1995

Volume

66

Issue

4

Start / End Page

349 / 364

Location

Germany

Related Subject Headings

  • Receptors, LDL
  • Rats, Wistar
  • Rats, Sprague-Dawley
  • Rats, Brattleboro
  • Rats
  • Rabbits
  • Microvilli
  • Microscopy, Electron
  • Membrane Glycoproteins
  • Male