Expression of the 71 kDa dystrophin isoform (Dp71) evaluated by gene targeting.
Publication
, Journal Article
Lumeng, CN; Hauser, M; Brown, V; Chamberlain, JS
Published in: Brain Res
May 29, 1999
To investigate the function of the major non-muscle dystrophin isoform, Dp71, we substituted a beta-galactosidase (betagal) reporter gene for Dp71 by homologous recombination in embryonic stem cells. Staining for betagal activity in chimeric mice revealed Dp71 promoter activity in glial cells in the CNS, in neurons of the inner nuclear and inner plexiform layers of the retina, and in the kidney tubules and collecting ducts. Our observations demonstrate that Dp71 is widely expressed in the adult CNS (retina, cerebellum, cerebral cortex, ependyma, and choroid) as well as the adult kidney epithelium and suggest a broad function for Dp71 in differentiated tissues.
Duke Scholars
Published In
Brain Res
DOI
ISSN
0006-8993
Publication Date
May 29, 1999
Volume
830
Issue
1
Start / End Page
174 / 178
Location
Netherlands
Related Subject Headings
- beta-Galactosidase
- Protein Isoforms
- Neurology & Neurosurgery
- Molecular Weight
- Mice, Inbred C57BL
- Mice
- In Situ Hybridization
- Genes, Reporter
- Gene Targeting
- Gene Expression Regulation
Citation
APA
Chicago
ICMJE
MLA
NLM
Lumeng, C. N., Hauser, M., Brown, V., & Chamberlain, J. S. (1999). Expression of the 71 kDa dystrophin isoform (Dp71) evaluated by gene targeting. Brain Res, 830(1), 174–178. https://doi.org/10.1016/s0006-8993(99)01201-9
Lumeng, C. N., M. Hauser, V. Brown, and J. S. Chamberlain. “Expression of the 71 kDa dystrophin isoform (Dp71) evaluated by gene targeting.” Brain Res 830, no. 1 (May 29, 1999): 174–78. https://doi.org/10.1016/s0006-8993(99)01201-9.
Lumeng CN, Hauser M, Brown V, Chamberlain JS. Expression of the 71 kDa dystrophin isoform (Dp71) evaluated by gene targeting. Brain Res. 1999 May 29;830(1):174–8.
Lumeng, C. N., et al. “Expression of the 71 kDa dystrophin isoform (Dp71) evaluated by gene targeting.” Brain Res, vol. 830, no. 1, May 1999, pp. 174–78. Pubmed, doi:10.1016/s0006-8993(99)01201-9.
Lumeng CN, Hauser M, Brown V, Chamberlain JS. Expression of the 71 kDa dystrophin isoform (Dp71) evaluated by gene targeting. Brain Res. 1999 May 29;830(1):174–178.
Published In
Brain Res
DOI
ISSN
0006-8993
Publication Date
May 29, 1999
Volume
830
Issue
1
Start / End Page
174 / 178
Location
Netherlands
Related Subject Headings
- beta-Galactosidase
- Protein Isoforms
- Neurology & Neurosurgery
- Molecular Weight
- Mice, Inbred C57BL
- Mice
- In Situ Hybridization
- Genes, Reporter
- Gene Targeting
- Gene Expression Regulation