A role for cFLIP in B cell proliferation and stress MAPK regulation.
Fas/Apo-1 signals through the FADD (Fas-associated death domain) adaptor protein, which recruits and activates the apical caspase 8 and leads to apoptosis. Cellular FLIP (cFLIP) is a homolog of caspase 8 and is also capable of binding to FADD. Previous studies suggest that cFLIP could either enhance or inhibit apoptosis and lead to NF-kappaB and Erk1/2 activation. Like FADD or caspase 8 deficiency, a lack of cFLIP disrupts embryogenesis and T cell proliferation. It has been demonstrated that B cells lacking either FADD or caspase 8 were defective in both Fas-induced apoptosis and TLR-induced proliferation, which indicates that these death-inducing proteins have an additional role in regulating innate immunity. To analyze the function of cFLIP in B cells, conditional deletion of cFLIP was induced by using CD19(Cre). The resulting B cell-specific cFLIP-deficient mice were found to have reduced numbers of peripheral B cells that were hypersensitive to Fas-induced apoptosis and impaired in proliferation induced by TLRs and the BCR. Furthermore, there was aberrant expression of costimulatory proteins and activation markers in cFLIP-deficient B cells. Whereas LPS-induced activation of NF-kappaB and Erk1/2 appears to be unaffected, p38 and Jnk were spontaneously activated and hyperinduced in cFLIP-deficient B cells. Therefore, these data revealed novel functions of cFLIP in B cells.
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Related Subject Headings
- p38 Mitogen-Activated Protein Kinases
- Mitogen-Activated Protein Kinase 9
- Mice, Transgenic
- Mice, Knockout
- Mice
- Immunology
- Hematopoietic Stem Cells
- Cell Proliferation
- Cell Differentiation
- CASP8 and FADD-Like Apoptosis Regulating Protein
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- p38 Mitogen-Activated Protein Kinases
- Mitogen-Activated Protein Kinase 9
- Mice, Transgenic
- Mice, Knockout
- Mice
- Immunology
- Hematopoietic Stem Cells
- Cell Proliferation
- Cell Differentiation
- CASP8 and FADD-Like Apoptosis Regulating Protein