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Toll-like receptor 2-dependent and -independent activation of macrophages by group B streptococci.

Publication ,  Journal Article
Draper, DW; Bethea, HN; He, Y-W
Published in: Immunol Lett
February 15, 2006

Group B streptococcus (GBS), a capsulated gram-positive bacterium, is a major cause of newborn infections. Although the innate immune receptor Toll-like receptor (TLR) 2 has been shown to primarily recognize gram-positive bacterial products, the production of TNF by macrophages treated with heat-killed GBS (HK-GBS) does not depend on TLR2. In this report, we have characterized HK-GBS-induced activation of macrophages derived from wildtype and TLR2-deficient mice. Microarray analysis demonstrated that HK-GBS activation of macrophages induces both TLR2-independent and -dependent signals. While the expression of a major fraction of genes in macrophages induced by HK-GBS does not depend on TLR2, induction of several important molecules involved in host innate immunity such as IL-6, IL-1beta, and lipocalin 2 is severely impaired in the absence of TLR2 signaling. Furthermore, we show that HK-GBS utilizes centrifugation sensitive components to induce rapid activation of TLR2(-/-) macrophages and that HK-GBS-induced activation of macrophages is not mediated through its genomic DNA. Together, our results demonstrate that HK-GBS induces TLR2-dependent antimicrobial gene activation and provide further understanding of the molecular basis of host innate response to GBS infection.

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Published In

Immunol Lett

DOI

ISSN

0165-2478

Publication Date

February 15, 2006

Volume

102

Issue

2

Start / End Page

202 / 214

Location

Netherlands

Related Subject Headings

  • Up-Regulation
  • Tumor Necrosis Factor-alpha
  • Toll-Like Receptor 2
  • Streptococcus agalactiae
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Macrophage Activation
  • Inflammation
  • Immunology
 

Citation

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Draper, D. W., Bethea, H. N., & He, Y.-W. (2006). Toll-like receptor 2-dependent and -independent activation of macrophages by group B streptococci. Immunol Lett, 102(2), 202–214. https://doi.org/10.1016/j.imlet.2005.09.005
Draper, David W., Heather N. Bethea, and You-Wen He. “Toll-like receptor 2-dependent and -independent activation of macrophages by group B streptococci.Immunol Lett 102, no. 2 (February 15, 2006): 202–14. https://doi.org/10.1016/j.imlet.2005.09.005.
Draper DW, Bethea HN, He Y-W. Toll-like receptor 2-dependent and -independent activation of macrophages by group B streptococci. Immunol Lett. 2006 Feb 15;102(2):202–14.
Draper, David W., et al. “Toll-like receptor 2-dependent and -independent activation of macrophages by group B streptococci.Immunol Lett, vol. 102, no. 2, Feb. 2006, pp. 202–14. Pubmed, doi:10.1016/j.imlet.2005.09.005.
Draper DW, Bethea HN, He Y-W. Toll-like receptor 2-dependent and -independent activation of macrophages by group B streptococci. Immunol Lett. 2006 Feb 15;102(2):202–214.
Journal cover image

Published In

Immunol Lett

DOI

ISSN

0165-2478

Publication Date

February 15, 2006

Volume

102

Issue

2

Start / End Page

202 / 214

Location

Netherlands

Related Subject Headings

  • Up-Regulation
  • Tumor Necrosis Factor-alpha
  • Toll-Like Receptor 2
  • Streptococcus agalactiae
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Macrophage Activation
  • Inflammation
  • Immunology