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Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms.

Publication ,  Journal Article
Viant, MR; Pincetich, CA; Walton, JH; Tjeerdema, RS; Hinton, DE
Published in: Marine environmental research
September 2002

In vivo nuclear magnetic resonance spectroscopy (NMR) is a powerful technique for characterizing the sublethal actions of physical and chemical stressors in live, intact organisms. In particular, 31P NMR is ideal for observing perturbations to cellular energetics since critical metabolite concentrations, including phosphagens, ATP and inorganic phosphate (Pi), can be measured non-invasively and in real time. This technique's versatility is demonstrated not only in the diversity of organisms that can be studied, but also in its broad-ranging applicability to environmental research. Illustrative studies include the actions of copper in adult red abalone (Haliotis rufescens) and changes in energetically important metabolites in developing medaka embryos (Oryzias latipes). Advantages and disadvantages of in vivo NMR will be discussed.

Duke Scholars

Published In

Marine environmental research

DOI

EISSN

1879-0291

ISSN

0141-1136

Publication Date

September 2002

Volume

54

Issue

3-5

Start / End Page

553 / 557

Related Subject Headings

  • Water Pollutants
  • Phosphates
  • Oryzias
  • Mollusca
  • Marine Biology & Hydrobiology
  • Magnetic Resonance Spectroscopy
  • Energy Metabolism
  • Embryo, Nonmammalian
  • Copper
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Viant, M. R., Pincetich, C. A., Walton, J. H., Tjeerdema, R. S., & Hinton, D. E. (2002). Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms. Marine Environmental Research, 54(3–5), 553–557. https://doi.org/10.1016/s0141-1136(02)00193-9
Viant, Mark R., Christopher A. Pincetich, Jeffrey H. Walton, Ronald S. Tjeerdema, and David E. Hinton. “Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms.Marine Environmental Research 54, no. 3–5 (September 2002): 553–57. https://doi.org/10.1016/s0141-1136(02)00193-9.
Viant MR, Pincetich CA, Walton JH, Tjeerdema RS, Hinton DE. Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms. Marine environmental research. 2002 Sep;54(3–5):553–7.
Viant, Mark R., et al. “Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms.Marine Environmental Research, vol. 54, no. 3–5, Sept. 2002, pp. 553–57. Epmc, doi:10.1016/s0141-1136(02)00193-9.
Viant MR, Pincetich CA, Walton JH, Tjeerdema RS, Hinton DE. Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms. Marine environmental research. 2002 Sep;54(3–5):553–557.
Journal cover image

Published In

Marine environmental research

DOI

EISSN

1879-0291

ISSN

0141-1136

Publication Date

September 2002

Volume

54

Issue

3-5

Start / End Page

553 / 557

Related Subject Headings

  • Water Pollutants
  • Phosphates
  • Oryzias
  • Mollusca
  • Marine Biology & Hydrobiology
  • Magnetic Resonance Spectroscopy
  • Energy Metabolism
  • Embryo, Nonmammalian
  • Copper
  • Animals