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Toxic responses of medaka, d-rR strain, to polychlorinated naphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life- cycle assessment

Publication ,  Journal Article
Villalobos, SA; Papoulias, DM; Meadows, J; Blankenship, AL; Pastva, SD; Kannan, K; Hinton, DE; Tillitt, DE; Giesy, JP
Published in: Environmental Toxicology and Chemistry
January 1, 2000

Polychlorinated naphthalenes (PCNs) are organic compounds with some chemical properties and uses similar to polychlorinated biphenyls. Polychlorinated naphthalenes have been detected in biota from certain aquatic environments. The toxicities of several PCN technical mixtures (Halowax) to medaka (Oryzias latipes) were determined by use of an embryo nanoinjection method. Medaka eggs (early gastrula) were injected with 0.5 nl of triolein (vehicle control) or 0.5 nl of four to five graded doses (0.3-30 ng/egg) of Halowax 1014, Halowax 1013, or Halowax 1051 in triolein. Following exposure, embryos developed, and fry were reared to sexual maturity (4 months), at which time they were euthanized. Responses were evaluated as early life stage (ELS) and early adult life stage (EALS) assessments. For ELS, lethality and sublethal alterations in embryos and larvae (<16 d old), such as craniofacial, cardiovascular, and myoskeletal deformities and abnormal or delayed hatch, were monitored for the first 9 d, and a dose severity index was computed. The EALS assessment examined the survival of 16-d-old larvae until early adulthood (123 ± 3 d old), including gonadosomatic index (GSI) and morphometry. Halowax 1014 was found to be the most toxic mixture (LD50 4.2 ng/egg), whereas Halowax 1013 and 1051 were significantly less toxic (LD50s could not be determined). The gonadosomatic index of females was significantly less in fish dosed with Halowax 1014 or 1051. The LD50 for medaka embryos nanoinjected with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is about 0.75 pg/egg. Thus, Halowax 1014 was 5,585-fold less potent than TCDD. For Halowax 1014, ELS assessments accurately predicted the results of EALS assessments.

Duke Scholars

Published In

Environmental Toxicology and Chemistry

DOI

ISSN

0730-7268

Publication Date

January 1, 2000

Volume

19

Issue

2

Start / End Page

432 / 440

Related Subject Headings

  • Environmental Sciences
  • 41 Environmental sciences
  • 34 Chemical sciences
  • 31 Biological sciences
  • 06 Biological Sciences
  • 05 Environmental Sciences
  • 03 Chemical Sciences
 

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Villalobos, S. A., Papoulias, D. M., Meadows, J., Blankenship, A. L., Pastva, S. D., Kannan, K., … Giesy, J. P. (2000). Toxic responses of medaka, d-rR strain, to polychlorinated naphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life- cycle assessment. Environmental Toxicology and Chemistry, 19(2), 432–440. https://doi.org/10.1002/etc.5620190224
Villalobos, S. A., D. M. Papoulias, J. Meadows, A. L. Blankenship, S. D. Pastva, K. Kannan, D. E. Hinton, D. E. Tillitt, and J. P. Giesy. “Toxic responses of medaka, d-rR strain, to polychlorinated naphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life- cycle assessment.” Environmental Toxicology and Chemistry 19, no. 2 (January 1, 2000): 432–40. https://doi.org/10.1002/etc.5620190224.
Villalobos SA, Papoulias DM, Meadows J, Blankenship AL, Pastva SD, Kannan K, et al. Toxic responses of medaka, d-rR strain, to polychlorinated naphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life- cycle assessment. Environmental Toxicology and Chemistry. 2000 Jan 1;19(2):432–40.
Villalobos, S. A., et al. “Toxic responses of medaka, d-rR strain, to polychlorinated naphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life- cycle assessment.” Environmental Toxicology and Chemistry, vol. 19, no. 2, Jan. 2000, pp. 432–40. Scopus, doi:10.1002/etc.5620190224.
Villalobos SA, Papoulias DM, Meadows J, Blankenship AL, Pastva SD, Kannan K, Hinton DE, Tillitt DE, Giesy JP. Toxic responses of medaka, d-rR strain, to polychlorinated naphthalene mixtures after embryonic exposure by in ovo nanoinjection: A partial life- cycle assessment. Environmental Toxicology and Chemistry. 2000 Jan 1;19(2):432–440.
Journal cover image

Published In

Environmental Toxicology and Chemistry

DOI

ISSN

0730-7268

Publication Date

January 1, 2000

Volume

19

Issue

2

Start / End Page

432 / 440

Related Subject Headings

  • Environmental Sciences
  • 41 Environmental sciences
  • 34 Chemical sciences
  • 31 Biological sciences
  • 06 Biological Sciences
  • 05 Environmental Sciences
  • 03 Chemical Sciences