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In vitro development of inner cell masses isolated from t0/t0 and tW5/tW5 mouse embryos.

Publication ,  Journal Article
Hogan, B; Spiegelman, M; Bennett, D
Published in: J Embryol Exp Morphol
December 1980

Inner cell masses (ICMs) isolated immunosurgically from mouse blastocysts segregating the homozygous lethal mutants t0/t0 and tW5/tW5 were cultured in vitro. Presumed t0/t0 ICMs fail to grow after three days in culture (equivalent gestational day 7.5) when they consist of an outer layer of endoderm cells surrounding about 30 epiblast cells. Presumed homozygous tW5/tW5 ICMs develop to a more advanced stage in culture and on the seventh day (equivalent gestational day 11.5) consist of an inner core of disorganized ectoderm cells with a small proamniotic cavity, surrounded by multiple layers of endoderm cells.

Duke Scholars

Published In

J Embryol Exp Morphol

ISSN

0022-0752

Publication Date

December 1980

Volume

60

Start / End Page

419 / 428

Location

England

Related Subject Headings

  • Mutation
  • Microscopy, Phase-Contrast
  • Mice, Mutant Strains
  • Mice
  • Homozygote
  • Genes, Lethal
  • Endoderm
  • Culture Techniques
  • Blastocyst
  • Animals
 

Citation

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Hogan, B., Spiegelman, M., & Bennett, D. (1980). In vitro development of inner cell masses isolated from t0/t0 and tW5/tW5 mouse embryos. J Embryol Exp Morphol, 60, 419–428.
Hogan, B., M. Spiegelman, and D. Bennett. “In vitro development of inner cell masses isolated from t0/t0 and tW5/tW5 mouse embryos.J Embryol Exp Morphol 60 (December 1980): 419–28.
Hogan B, Spiegelman M, Bennett D. In vitro development of inner cell masses isolated from t0/t0 and tW5/tW5 mouse embryos. J Embryol Exp Morphol. 1980 Dec;60:419–28.
Hogan, B., et al. “In vitro development of inner cell masses isolated from t0/t0 and tW5/tW5 mouse embryos.J Embryol Exp Morphol, vol. 60, Dec. 1980, pp. 419–28.
Hogan B, Spiegelman M, Bennett D. In vitro development of inner cell masses isolated from t0/t0 and tW5/tW5 mouse embryos. J Embryol Exp Morphol. 1980 Dec;60:419–428.

Published In

J Embryol Exp Morphol

ISSN

0022-0752

Publication Date

December 1980

Volume

60

Start / End Page

419 / 428

Location

England

Related Subject Headings

  • Mutation
  • Microscopy, Phase-Contrast
  • Mice, Mutant Strains
  • Mice
  • Homozygote
  • Genes, Lethal
  • Endoderm
  • Culture Techniques
  • Blastocyst
  • Animals