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Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins.

Publication ,  Journal Article
Hsu, DS; Zhao, X; Zhao, S; Kazantsev, A; Wang, RP; Todo, T; Wei, YF; Sancar, A
Published in: Biochemistry
November 5, 1996

Recently, a human cDNA clone with high sequence homology to the photolyase/blue-light photoreceptor family was identified. The putative protein encoded by this gene exhibited a strikingly high (48% identity) degree of homology to the Drosophila melanogaster (6-4) photolyase [Todo et al. (1996) Science 272, 109-112]. We have now identified a second human gene whose amino acid sequence displays 73% identity to the first one and have named the two genes CRY1 and CRY2, respectively. The corresponding proteins hCRY1 and hCRY2 were purified and characterized as maltose-binding fusion proteins. Similar to other members of the photolyase/blue-light photoreceptor family, both proteins were found to contain FAD and a pterin cofactor. Like the plant blue-light photoreceptors, both hCRY1 and hCRY2 lacked photolyase activity on the cyclobutane pyrimidine dimer and the (6-4) photoproduct. We conclude that these newly discovered members of the photolyase/photoreceptor family are not photolyases and instead may function as blue-light photoreceptors in humans.

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Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

November 5, 1996

Volume

35

Issue

44

Start / End Page

13871 / 13877

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Sequence Homology, Amino Acid
  • Recombinant Proteins
  • Receptors, G-Protein-Coupled
  • Plasmids
  • Photoreceptor Cells, Invertebrate
  • Photoreceptor Cells
  • Molecular Sequence Data
  • Humans
  • Flavoproteins
 

Citation

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Hsu, D. S., Zhao, X., Zhao, S., Kazantsev, A., Wang, R. P., Todo, T., … Sancar, A. (1996). Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins. Biochemistry, 35(44), 13871–13877. https://doi.org/10.1021/bi962209o
Hsu, D. S., X. Zhao, S. Zhao, A. Kazantsev, R. P. Wang, T. Todo, Y. F. Wei, and A. Sancar. “Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins.Biochemistry 35, no. 44 (November 5, 1996): 13871–77. https://doi.org/10.1021/bi962209o.
Hsu DS, Zhao X, Zhao S, Kazantsev A, Wang RP, Todo T, et al. Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins. Biochemistry. 1996 Nov 5;35(44):13871–7.
Hsu, D. S., et al. “Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins.Biochemistry, vol. 35, no. 44, Nov. 1996, pp. 13871–77. Pubmed, doi:10.1021/bi962209o.
Hsu DS, Zhao X, Zhao S, Kazantsev A, Wang RP, Todo T, Wei YF, Sancar A. Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins. Biochemistry. 1996 Nov 5;35(44):13871–13877.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

November 5, 1996

Volume

35

Issue

44

Start / End Page

13871 / 13877

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Sequence Homology, Amino Acid
  • Recombinant Proteins
  • Receptors, G-Protein-Coupled
  • Plasmids
  • Photoreceptor Cells, Invertebrate
  • Photoreceptor Cells
  • Molecular Sequence Data
  • Humans
  • Flavoproteins