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Development of a novel method for measuring in vivo breast epithelial cell proliferation in humans.

Publication ,  Journal Article
Misell, LM; Hwang, ES; Au, A; Esserman, L; Hellerstein, MK
Published in: Breast Cancer Res Treat
February 2005

Cell proliferation plays an important role in all stages of carcinogenesis. Currently, no safe, direct, in vivo method of measuring breast epithelial cell (BEC) proliferation rates in humans exists. Static immunohistochemical markers of cell proliferation, such as Ki-67 and PCNA indices, have technical limitations including high inter-lab variability, inaccuracy in the presence of agents that cause G1/S cell cycle block and inadequate sensitivity in post-menopausal women with low BEC proliferation rates. We describe here a safe, direct method of measuring BEC proliferation rates in vivo in women using heavy water ((2)H(2)O) labeling coupled with mass spectrometric analysis. Proliferation of normal and tumor BEC was measured from breast tissue biopsies in women undergoing mastectomy (n = 11) and normal BEC from healthy volunteers (n = 16). Women took heavy water (50-150 ml per day) for 1-4 weeks. Pre-menopausal women had significantly higher proliferation rates than post-menopausal women (0.7 +/- 0.1 versus 0.2 +/- 0.1 new cells per day, respectively), and tumor BEC had different proliferation rates than normal BEC from the same breast. The method is analytically reproducible and remains sensitive in the range of low proliferation rates. In summary, this novel method of measuring BEC proliferation in vivo holds promise for assessing the effects of anti-proliferative chemopreventive and chemotherapeutic agents.

Duke Scholars

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Published In

Breast Cancer Res Treat

DOI

ISSN

0167-6806

Publication Date

February 2005

Volume

89

Issue

3

Start / End Page

257 / 264

Location

Netherlands

Related Subject Headings

  • Water
  • Reproducibility of Results
  • Oncology & Carcinogenesis
  • Monocytes
  • Middle Aged
  • Menopause
  • Mass Spectrometry
  • Immunomagnetic Separation
  • Humans
  • Female
 

Citation

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Misell, L. M., Hwang, E. S., Au, A., Esserman, L., & Hellerstein, M. K. (2005). Development of a novel method for measuring in vivo breast epithelial cell proliferation in humans. Breast Cancer Res Treat, 89(3), 257–264. https://doi.org/10.1007/s10549-004-2228-5
Misell, Lisa M., E Shelley Hwang, Alfred Au, Laura Esserman, and Marc K. Hellerstein. “Development of a novel method for measuring in vivo breast epithelial cell proliferation in humans.Breast Cancer Res Treat 89, no. 3 (February 2005): 257–64. https://doi.org/10.1007/s10549-004-2228-5.
Misell LM, Hwang ES, Au A, Esserman L, Hellerstein MK. Development of a novel method for measuring in vivo breast epithelial cell proliferation in humans. Breast Cancer Res Treat. 2005 Feb;89(3):257–64.
Misell, Lisa M., et al. “Development of a novel method for measuring in vivo breast epithelial cell proliferation in humans.Breast Cancer Res Treat, vol. 89, no. 3, Feb. 2005, pp. 257–64. Pubmed, doi:10.1007/s10549-004-2228-5.
Misell LM, Hwang ES, Au A, Esserman L, Hellerstein MK. Development of a novel method for measuring in vivo breast epithelial cell proliferation in humans. Breast Cancer Res Treat. 2005 Feb;89(3):257–264.
Journal cover image

Published In

Breast Cancer Res Treat

DOI

ISSN

0167-6806

Publication Date

February 2005

Volume

89

Issue

3

Start / End Page

257 / 264

Location

Netherlands

Related Subject Headings

  • Water
  • Reproducibility of Results
  • Oncology & Carcinogenesis
  • Monocytes
  • Middle Aged
  • Menopause
  • Mass Spectrometry
  • Immunomagnetic Separation
  • Humans
  • Female