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Correlation of endoscopic optical coherence tomography with histology in the lower-GI tract.

Publication ,  Journal Article
Westphal, V; Rollins, AM; Willis, J; Sivak, MV; Izatt, JA
Published in: Gastrointestinal endoscopy
April 2005

Optical coherence tomography (OCT), a noninvasive optical imaging technique, provides high-resolution cross-sectional images of tissue microstructure. We developed a system for real-time endoscopic OCT (EOCT) of the human GI tract. During clinical trials, the structure of mucosa and submucosa, glands, blood vessels, pits, villi, and crypts was observed in a range of GI organs. Although EOCT images are thought to accurately depict actual histologic features, there are few data to support this assumption. Therefore, the present study correlated images acquired with an EOCT imaging system in vitro to corresponding histologic sections.EOCT images were obtained of fresh specimens of ileum, colon, and rectum that then were fixed in formalin and were processed for microscopic evaluation by using standard methods. The thickness of mucosa and of submucosa was determined for both EOCT images and histologic slides.The first hyper-reflective layer in the EOCT images was identified as mucosa. A close correlation (R 2=0.84) was observed between EOCT and histology. Furthermore, the submucosa and the muscularis propria could be identified as the next deepest hyporeflective band and a hyper-reflective layer, respectively, in EOCT images. The submucosa was found to be more compressible than mucosa, and its EOCT appearance was dependent on its content of adipose tissue.EOCT provides images that precisely correlate with the histologic structure of the mucosa and the submucosa of the GI tract.

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Published In

Gastrointestinal endoscopy

DOI

EISSN

1097-6779

ISSN

0016-5107

Publication Date

April 2005

Volume

61

Issue

4

Start / End Page

537 / 546

Related Subject Headings

  • Tomography, Optical Coherence
  • Reproducibility of Results
  • Rectum
  • Intestinal Mucosa
  • In Vitro Techniques
  • Image Processing, Computer-Assisted
  • Ileum
  • Humans
  • Gastroenterology & Hepatology
  • Endoscopy, Gastrointestinal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Westphal, V., Rollins, A. M., Willis, J., Sivak, M. V., & Izatt, J. A. (2005). Correlation of endoscopic optical coherence tomography with histology in the lower-GI tract. Gastrointestinal Endoscopy, 61(4), 537–546. https://doi.org/10.1016/s0016-5107(05)00084-2
Westphal, Volker, Andrew M. Rollins, Joseph Willis, Michael V. Sivak, and Joseph A. Izatt. “Correlation of endoscopic optical coherence tomography with histology in the lower-GI tract.Gastrointestinal Endoscopy 61, no. 4 (April 2005): 537–46. https://doi.org/10.1016/s0016-5107(05)00084-2.
Westphal V, Rollins AM, Willis J, Sivak MV, Izatt JA. Correlation of endoscopic optical coherence tomography with histology in the lower-GI tract. Gastrointestinal endoscopy. 2005 Apr;61(4):537–46.
Westphal, Volker, et al. “Correlation of endoscopic optical coherence tomography with histology in the lower-GI tract.Gastrointestinal Endoscopy, vol. 61, no. 4, Apr. 2005, pp. 537–46. Epmc, doi:10.1016/s0016-5107(05)00084-2.
Westphal V, Rollins AM, Willis J, Sivak MV, Izatt JA. Correlation of endoscopic optical coherence tomography with histology in the lower-GI tract. Gastrointestinal endoscopy. 2005 Apr;61(4):537–546.
Journal cover image

Published In

Gastrointestinal endoscopy

DOI

EISSN

1097-6779

ISSN

0016-5107

Publication Date

April 2005

Volume

61

Issue

4

Start / End Page

537 / 546

Related Subject Headings

  • Tomography, Optical Coherence
  • Reproducibility of Results
  • Rectum
  • Intestinal Mucosa
  • In Vitro Techniques
  • Image Processing, Computer-Assisted
  • Ileum
  • Humans
  • Gastroenterology & Hepatology
  • Endoscopy, Gastrointestinal