Skip to main content
Journal cover image

Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.

Publication ,  Journal Article
Takagi, M; Absalon, MJ; McLure, KG; Kastan, MB
Published in: Cell
October 7, 2005

Increases in p53 protein levels after DNA damage have largely been attributed to an increase in the half-life of p53 protein. Here we demonstrate that increased translation of p53 mRNA is also a critical step in the induction of p53 protein in irradiated cells. Ribosomal protein L26 (RPL26) and nucleolin were found to bind to the 5' untranslated region (UTR) of p53 mRNA and to control p53 translation and induction after DNA damage. RPL26 preferentially binds to the 5'UTR after DNA damage, and its overexpression enhances association of p53 mRNA with heavier polysomes, increases the rate of p53 translation, induces G1 cell-cycle arrest, and augments irradiation-induced apoptosis. Opposite effects were seen when RPL26 expression was inhibited. In contrast, nucleolin overexpression suppresses p53 translation and induction after DNA damage, whereas nucleolin downregulation promotes p53 expression. These findings demonstrate the importance of increased translation of p53 in DNA-damage responses and suggest critical roles for RPL26 and nucleolin in affecting p53 induction.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 7, 2005

Volume

123

Issue

1

Start / End Page

49 / 63

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tumor Suppressor Protein p53
  • Ribosomal Proteins
  • Regulatory Elements, Transcriptional
  • RNA-Binding Proteins
  • RNA, Messenger
  • Protein Biosynthesis
  • Phosphoproteins
  • Nucleolin
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Takagi, M., Absalon, M. J., McLure, K. G., & Kastan, M. B. (2005). Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin. Cell, 123(1), 49–63. https://doi.org/10.1016/j.cell.2005.07.034
Takagi, Masatoshi, Michael J. Absalon, Kevin G. McLure, and Michael B. Kastan. “Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.Cell 123, no. 1 (October 7, 2005): 49–63. https://doi.org/10.1016/j.cell.2005.07.034.
Takagi M, Absalon MJ, McLure KG, Kastan MB. Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin. Cell. 2005 Oct 7;123(1):49–63.
Takagi, Masatoshi, et al. “Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin.Cell, vol. 123, no. 1, Oct. 2005, pp. 49–63. Pubmed, doi:10.1016/j.cell.2005.07.034.
Takagi M, Absalon MJ, McLure KG, Kastan MB. Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin. Cell. 2005 Oct 7;123(1):49–63.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 7, 2005

Volume

123

Issue

1

Start / End Page

49 / 63

Location

United States

Related Subject Headings

  • Up-Regulation
  • Tumor Suppressor Protein p53
  • Ribosomal Proteins
  • Regulatory Elements, Transcriptional
  • RNA-Binding Proteins
  • RNA, Messenger
  • Protein Biosynthesis
  • Phosphoproteins
  • Nucleolin
  • Mice