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Analyzing cell cycle checkpoints after ionizing radiation.

Publication ,  Journal Article
Xu, B; Kastan, MB
Published in: Methods Mol Biol
2004

Several methods to measure cell cycle perturbation after exposure to ionizing radiation are presented in this chapter. These methods include the G1 checkpoint assay by 5' bromode-oxyuridine (BrdUrd) labeling followed by flow cytometric analysis, the S-phase checkpoint assay by measuring DNA synthesis in response to ionizing radiation, and the G2/M checkpoint assay by quantitating histone H3 phosphorylation after ionizing radiation.

Duke Scholars

Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2004

Volume

281

Start / End Page

283 / 292

Location

United States

Related Subject Headings

  • Radiation-Sensitizing Agents
  • Radiation, Ionizing
  • Phosphorylation
  • Humans
  • Histones
  • Hela Cells
  • HeLa Cells
  • Flow Cytometry
  • Developmental Biology
  • DNA
 

Citation

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Xu, B., & Kastan, M. B. (2004). Analyzing cell cycle checkpoints after ionizing radiation. Methods Mol Biol, 281, 283–292. https://doi.org/10.1385/1-59259-811-0:283
Xu, Bo, and Michael B. Kastan. “Analyzing cell cycle checkpoints after ionizing radiation.Methods Mol Biol 281 (2004): 283–92. https://doi.org/10.1385/1-59259-811-0:283.
Xu B, Kastan MB. Analyzing cell cycle checkpoints after ionizing radiation. Methods Mol Biol. 2004;281:283–92.
Xu, Bo, and Michael B. Kastan. “Analyzing cell cycle checkpoints after ionizing radiation.Methods Mol Biol, vol. 281, 2004, pp. 283–92. Pubmed, doi:10.1385/1-59259-811-0:283.
Xu B, Kastan MB. Analyzing cell cycle checkpoints after ionizing radiation. Methods Mol Biol. 2004;281:283–292.

Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2004

Volume

281

Start / End Page

283 / 292

Location

United States

Related Subject Headings

  • Radiation-Sensitizing Agents
  • Radiation, Ionizing
  • Phosphorylation
  • Humans
  • Histones
  • Hela Cells
  • HeLa Cells
  • Flow Cytometry
  • Developmental Biology
  • DNA