Analyzing cell cycle checkpoints after ionizing radiation.
Publication
, Journal Article
Xu, B; Kastan, MB
Published in: Methods Mol Biol
2004
Several methods to measure cell cycle perturbation after exposure to ionizing radiation are presented in this chapter. These methods include the G1 checkpoint assay by 5' bromode-oxyuridine (BrdUrd) labeling followed by flow cytometric analysis, the S-phase checkpoint assay by measuring DNA synthesis in response to ionizing radiation, and the G2/M checkpoint assay by quantitating histone H3 phosphorylation after ionizing radiation.
Duke Scholars
Published In
Methods Mol Biol
DOI
ISSN
1064-3745
Publication Date
2004
Volume
281
Start / End Page
283 / 292
Location
United States
Related Subject Headings
- Radiation-Sensitizing Agents
- Radiation, Ionizing
- Phosphorylation
- Humans
- Histones
- Hela Cells
- HeLa Cells
- Flow Cytometry
- Developmental Biology
- DNA
Citation
APA
Chicago
ICMJE
MLA
NLM
Xu, B., & Kastan, M. B. (2004). Analyzing cell cycle checkpoints after ionizing radiation. Methods Mol Biol, 281, 283–292. https://doi.org/10.1385/1-59259-811-0:283
Xu, Bo, and Michael B. Kastan. “Analyzing cell cycle checkpoints after ionizing radiation.” Methods Mol Biol 281 (2004): 283–92. https://doi.org/10.1385/1-59259-811-0:283.
Xu B, Kastan MB. Analyzing cell cycle checkpoints after ionizing radiation. Methods Mol Biol. 2004;281:283–92.
Xu, Bo, and Michael B. Kastan. “Analyzing cell cycle checkpoints after ionizing radiation.” Methods Mol Biol, vol. 281, 2004, pp. 283–92. Pubmed, doi:10.1385/1-59259-811-0:283.
Xu B, Kastan MB. Analyzing cell cycle checkpoints after ionizing radiation. Methods Mol Biol. 2004;281:283–292.
Published In
Methods Mol Biol
DOI
ISSN
1064-3745
Publication Date
2004
Volume
281
Start / End Page
283 / 292
Location
United States
Related Subject Headings
- Radiation-Sensitizing Agents
- Radiation, Ionizing
- Phosphorylation
- Humans
- Histones
- Hela Cells
- HeLa Cells
- Flow Cytometry
- Developmental Biology
- DNA