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Activation of the ATM kinase by ionizing radiation and phosphorylation of p53.

Publication ,  Journal Article
Canman, CE; Lim, DS; Cimprich, KA; Taya, Y; Tamai, K; Sakaguchi, K; Appella, E; Kastan, MB; Siliciano, JD
Published in: Science
September 11, 1998

The p53 tumor suppressor protein is activated and phosphorylated on serine-15 in response to various DNA damaging agents. The gene product mutated in ataxia telangiectasia, ATM, acts upstream of p53 in a signal transduction pathway initiated by ionizing radiation. Immunoprecipitated ATM had intrinsic protein kinase activity and phosphorylated p53 on serine-15 in a manganese-dependent manner. Ionizing radiation, but not ultraviolet radiation, rapidly enhanced this p53-directed kinase activity of endogenous ATM. These observations, along with the fact that phosphorylation of p53 on serine-15 in response to ionizing radiation is reduced in ataxia telangiectasia cells, suggest that ATM is a protein kinase that phosphorylates p53 in vivo.

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Published In

Science

DOI

ISSN

0036-8075

Publication Date

September 11, 1998

Volume

281

Issue

5383

Start / End Page

1677 / 1679

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Tumor Suppressor Proteins
  • Tumor Suppressor Protein p53
  • Transfection
  • Signal Transduction
  • Recombinant Proteins
  • Recombinant Fusion Proteins
  • Radiation, Ionizing
  • Proteins
  • Protein Serine-Threonine Kinases
 

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Canman, C. E., Lim, D. S., Cimprich, K. A., Taya, Y., Tamai, K., Sakaguchi, K., … Siliciano, J. D. (1998). Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science, 281(5383), 1677–1679. https://doi.org/10.1126/science.281.5383.1677
Canman, C. E., D. S. Lim, K. A. Cimprich, Y. Taya, K. Tamai, K. Sakaguchi, E. Appella, M. B. Kastan, and J. D. Siliciano. “Activation of the ATM kinase by ionizing radiation and phosphorylation of p53.Science 281, no. 5383 (September 11, 1998): 1677–79. https://doi.org/10.1126/science.281.5383.1677.
Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, et al. Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science. 1998 Sep 11;281(5383):1677–9.
Canman, C. E., et al. “Activation of the ATM kinase by ionizing radiation and phosphorylation of p53.Science, vol. 281, no. 5383, Sept. 1998, pp. 1677–79. Pubmed, doi:10.1126/science.281.5383.1677.
Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, Appella E, Kastan MB, Siliciano JD. Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science. 1998 Sep 11;281(5383):1677–1679.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

September 11, 1998

Volume

281

Issue

5383

Start / End Page

1677 / 1679

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Tumor Suppressor Proteins
  • Tumor Suppressor Protein p53
  • Transfection
  • Signal Transduction
  • Recombinant Proteins
  • Recombinant Fusion Proteins
  • Radiation, Ionizing
  • Proteins
  • Protein Serine-Threonine Kinases