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Fragments of ATM which have dominant-negative or complementing activity.

Publication ,  Journal Article
Morgan, SE; Lovly, C; Pandita, TK; Shiloh, Y; Kastan, MB
Published in: Mol Cell Biol
April 1997

The ATM protein has been implicated in pathways controlling cell cycle checkpoints, radiosensitivity, genetic instability, and aging. Expression of ATM fragments containing a leucine zipper motif in a human tumor cell line abrogated the S-phase checkpoint after ionizing irradiation and enhanced radiosensitivity and chromosomal breakage. These fragments did not abrogate irradiation-induced G1 or G2 checkpoints, suggesting that cell cycle checkpoint defects alone cannot account for chromosomal instability in ataxia telangiectasia (AT) cells. Expression of the carboxy-terminal portion of ATM, which contains the PI-3 kinase domain, complemented radiosensitivity and the S-phase checkpoint and reduced chromosomal breakage after irradiation in AT cells. These observations suggest that ATM function is dependent on interactions with itself or other proteins through the leucine zipper region and that the PI-3 kinase domain contains much of the significant activity of ATM.

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Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

April 1997

Volume

17

Issue

4

Start / End Page

2020 / 2029

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Radiation Tolerance
  • Proteins
  • Protein Serine-Threonine Kinases
  • Phosphotransferases (Alcohol Group Acceptor)
  • Phosphatidylinositol 3-Kinases
  • Phenotype
  • Peptide Fragments
  • Leucine Zippers
  • Humans
 

Citation

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Morgan, S. E., Lovly, C., Pandita, T. K., Shiloh, Y., & Kastan, M. B. (1997). Fragments of ATM which have dominant-negative or complementing activity. Mol Cell Biol, 17(4), 2020–2029. https://doi.org/10.1128/MCB.17.4.2020
Morgan, S. E., C. Lovly, T. K. Pandita, Y. Shiloh, and M. B. Kastan. “Fragments of ATM which have dominant-negative or complementing activity.Mol Cell Biol 17, no. 4 (April 1997): 2020–29. https://doi.org/10.1128/MCB.17.4.2020.
Morgan SE, Lovly C, Pandita TK, Shiloh Y, Kastan MB. Fragments of ATM which have dominant-negative or complementing activity. Mol Cell Biol. 1997 Apr;17(4):2020–9.
Morgan, S. E., et al. “Fragments of ATM which have dominant-negative or complementing activity.Mol Cell Biol, vol. 17, no. 4, Apr. 1997, pp. 2020–29. Pubmed, doi:10.1128/MCB.17.4.2020.
Morgan SE, Lovly C, Pandita TK, Shiloh Y, Kastan MB. Fragments of ATM which have dominant-negative or complementing activity. Mol Cell Biol. 1997 Apr;17(4):2020–2029.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

April 1997

Volume

17

Issue

4

Start / End Page

2020 / 2029

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Radiation Tolerance
  • Proteins
  • Protein Serine-Threonine Kinases
  • Phosphotransferases (Alcohol Group Acceptor)
  • Phosphatidylinositol 3-Kinases
  • Phenotype
  • Peptide Fragments
  • Leucine Zippers
  • Humans