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Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage.

Publication ,  Journal Article
Slichenmyer, WJ; Nelson, WG; Slebos, RJ; Kastan, MB
Published in: Cancer Res
September 15, 1993

Cell cycle checkpoints regulate progression through the cell cycle. In yeast, loss of the G2 checkpoint by mutation of the rad9 gene results in increased genetic instability as well as increased sensitivity to ionizing radiation. In contrast, comparing clonogenic survival of cells which are isogeneic except for p53 functional status, we find that loss of a G1 checkpoint in mammalian cells is not associated with increased sensitivity to the lethal effects of ionizing radiation or a topoisomerase I inhibitor, camptothecin. These results indicate that increased sensitivity to DNA-damaging agents is not necessarily a defining feature of a mammalian cell cycle checkpoint. Furthermore, in light of a recent link of p53 function to radiation-induced apoptosis in hematopoietic cells, these observations suggest that p53-dependent apoptosis is a cell type-specific phenomenon and thus predict that the biological consequences of loss of p53 function will be cell type specific.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

September 15, 1993

Volume

53

Issue

18

Start / End Page

4164 / 4168

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Radiation Tolerance
  • Oncology & Carcinogenesis
  • Mutation
  • Humans
  • Genes, p53
  • G1 Phase
  • DNA Damage
  • Cell Survival
  • Camptothecin
 

Citation

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MLA
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Slichenmyer, W. J., Nelson, W. G., Slebos, R. J., & Kastan, M. B. (1993). Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage. Cancer Res, 53(18), 4164–4168.
Slichenmyer, W. J., W. G. Nelson, R. J. Slebos, and M. B. Kastan. “Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage.Cancer Res 53, no. 18 (September 15, 1993): 4164–68.
Slichenmyer WJ, Nelson WG, Slebos RJ, Kastan MB. Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage. Cancer Res. 1993 Sep 15;53(18):4164–8.
Slichenmyer, W. J., et al. “Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage.Cancer Res, vol. 53, no. 18, Sept. 1993, pp. 4164–68.
Slichenmyer WJ, Nelson WG, Slebos RJ, Kastan MB. Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage. Cancer Res. 1993 Sep 15;53(18):4164–4168.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

September 15, 1993

Volume

53

Issue

18

Start / End Page

4164 / 4168

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Radiation Tolerance
  • Oncology & Carcinogenesis
  • Mutation
  • Humans
  • Genes, p53
  • G1 Phase
  • DNA Damage
  • Cell Survival
  • Camptothecin