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Species differences in susceptibility to 1,3-dinitrobenzene-induced testicular toxicity and methemoglobinemia.

Publication ,  Journal Article
Obasaju, MF; Katz, DF; Miller, MG
Published in: Fundamental and applied toxicology : official journal of the Society of Toxicology
February 1991

The testicular toxicity and methemoglobinemia induced by 1,3-dinitrobenzene (1,3-DNB) was compared in two species, the Sprague-Dawley rat and the golden Syrian hamster. A marked difference in susceptibility to both endpoints of toxicity was observed. The hamster showed no testicular lesions at dose levels up to 50 mg/kg whereas, as previously reported by others, damage to rat testicular tubules in later stages of spermatogenesis was readily apparent at a 25 mg/kg dose level. Similarly, administration of 1,3-DNB induced substantially less methemoglobinemia in the hamster than in the rat. For example, at the 25 mg/kg dose level peak levels of methemoglobin in the hamster were 15% compared with 80% in the rat. Mortality in the rat also occurred at lower doses than in the hamster (50 vs 100 mg/kg, respectively). In in vitro studies, the capacity of 1,3-DNB and 1,3-DNB metabolites (nitroaniline, nitroacetanilide, aminoacetanilide, diacetamidobenzene) to induce methemoglobinemia was examined in suspensions of red blood cells obtained from both species. Only 1,3-DNB caused the formation of methemoglobin and rat red blood cells were twice as sensitive as hamster red blood cells. The species difference in susceptibility to both methemoglobinemia and testicular toxicity could indicate differences in 1,3-DNB clearance and/or formation of toxic metabolites. Additional metabolic work is under way. This study demonstrates that the hamster is more resistant than the rat to the testicular lesion and methemoglobinemia induced by 1,3-DNB.

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Published In

Fundamental and applied toxicology : official journal of the Society of Toxicology

DOI

ISSN

0272-0590

Publication Date

February 1991

Volume

16

Issue

2

Start / End Page

257 / 266

Related Subject Headings

  • Toxicology
  • Testis
  • Species Specificity
  • Rats, Inbred Strains
  • Rats
  • Methemoglobinemia
  • Mesocricetus
  • Male
  • In Vitro Techniques
  • Disease Susceptibility
 

Citation

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Obasaju, M. F., Katz, D. F., & Miller, M. G. (1991). Species differences in susceptibility to 1,3-dinitrobenzene-induced testicular toxicity and methemoglobinemia. Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology, 16(2), 257–266. https://doi.org/10.1016/0272-0590(91)90110-p
Obasaju, M. F., D. F. Katz, and M. G. Miller. “Species differences in susceptibility to 1,3-dinitrobenzene-induced testicular toxicity and methemoglobinemia.Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology 16, no. 2 (February 1991): 257–66. https://doi.org/10.1016/0272-0590(91)90110-p.
Obasaju MF, Katz DF, Miller MG. Species differences in susceptibility to 1,3-dinitrobenzene-induced testicular toxicity and methemoglobinemia. Fundamental and applied toxicology : official journal of the Society of Toxicology. 1991 Feb;16(2):257–66.
Obasaju, M. F., et al. “Species differences in susceptibility to 1,3-dinitrobenzene-induced testicular toxicity and methemoglobinemia.Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology, vol. 16, no. 2, Feb. 1991, pp. 257–66. Epmc, doi:10.1016/0272-0590(91)90110-p.
Obasaju MF, Katz DF, Miller MG. Species differences in susceptibility to 1,3-dinitrobenzene-induced testicular toxicity and methemoglobinemia. Fundamental and applied toxicology : official journal of the Society of Toxicology. 1991 Feb;16(2):257–266.

Published In

Fundamental and applied toxicology : official journal of the Society of Toxicology

DOI

ISSN

0272-0590

Publication Date

February 1991

Volume

16

Issue

2

Start / End Page

257 / 266

Related Subject Headings

  • Toxicology
  • Testis
  • Species Specificity
  • Rats, Inbred Strains
  • Rats
  • Methemoglobinemia
  • Mesocricetus
  • Male
  • In Vitro Techniques
  • Disease Susceptibility