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Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR.

Publication ,  Journal Article
Jain, RG; Andrews, LG; McGowan, KM; Gao, F; Keene, JD; Pekala, PP
Published in: Nucleic Acids Symp Ser
1995

Hel-N1, is an RRM protein which is a mammalian homologue of the Drosophila melanogaster RNA binding protein, ELAV (embryonic lethal abnormal vision). Hel-N1 binds to RNA containing short stretches of uridylates similar to those found in the 3' untranslated regions (3'-UTRs) of oncoprotein and cytokine mRNAs. The GLUT1 glucose transporter has an extensive 3' UTR that is AU-rich reminiscent of the 3'UTR of an oncogene mRNA. An in vitro RNA binding assay using Hel-N1 demonstrated binding to a specific portion of the GLUT1 3'UTR. Analysis of the folding pattern of this region depicted the retention of a stem loop structure, wherein the loop is composed of a stretch of uridylates. To further analyze the potential function of Hel-N1, stable transfectants were made in the 3T3-L1 cell line. The transfectants have been characterized, and the presence of the Hel-N1 DNA and protein verified. Data indicate Hel-N1 is a ligand for GLUT1 and its binding affects the stability and translatability of the GLUT1 message.

Duke Scholars

Published In

Nucleic Acids Symp Ser

ISSN

0261-3166

Publication Date

1995

Issue

33

Start / End Page

209 / 211

Location

England

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • RNA-Binding Proteins
  • RNA, Messenger
  • Nucleic Acid Conformation
  • Nerve Tissue Proteins
  • Monosaccharide Transport Proteins
  • Molecular Sequence Data
  • Mice
  • Ligands
  • Humans
 

Citation

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MLA
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Jain, R. G., Andrews, L. G., McGowan, K. M., Gao, F., Keene, J. D., & Pekala, P. P. (1995). Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR. Nucleic Acids Symp Ser, (33), 209–211.
Jain, R. G., L. G. Andrews, K. M. McGowan, F. Gao, J. D. Keene, and P. P. Pekala. “Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR.Nucleic Acids Symp Ser, no. 33 (1995): 209–11.
Jain RG, Andrews LG, McGowan KM, Gao F, Keene JD, Pekala PP. Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR. Nucleic Acids Symp Ser. 1995;(33):209–11.
Jain, R. G., et al. “Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR.Nucleic Acids Symp Ser, no. 33, 1995, pp. 209–11.
Jain RG, Andrews LG, McGowan KM, Gao F, Keene JD, Pekala PP. Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR. Nucleic Acids Symp Ser. 1995;(33):209–211.

Published In

Nucleic Acids Symp Ser

ISSN

0261-3166

Publication Date

1995

Issue

33

Start / End Page

209 / 211

Location

England

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • RNA-Binding Proteins
  • RNA, Messenger
  • Nucleic Acid Conformation
  • Nerve Tissue Proteins
  • Monosaccharide Transport Proteins
  • Molecular Sequence Data
  • Mice
  • Ligands
  • Humans