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Antigenicity of a recombinant Ro (SS-A) fusion protein.

Publication ,  Journal Article
James, JA; Dickey, WD; Fujisaku, A; O'Brien, CA; Deutscher, SL; Keene, JD; Harley, JB
Published in: Arthritis Rheum
January 1990

The antigenicity of the 60-kd human Ro (SS-A) synthesized in vitro from its complementary DNA as a beta-galactosidase fusion protein (beta-gal-Ro) was evaluated by Western blotting. In this analysis, almost all the anti-Ro (SS-A)-positive sera that bound beta-gal-Ro also bound affinity-purified 60-kd human Ro (SS-A) (P less than 0.005). Three of the 27 anti-Ro (SS-A) precipitin-positive sera, however, did not show reactivity on Western blot analysis, which suggests that in some sera, antigenicity to Ro (SS-A) is destroyed by denaturation. Of the 22 sera that were reactive with beta-gal-Ro, 2 were not reactive with affinity-purified human Ro (SS-A). Two serum samples that did not react with beta-gal-Ro were also reactive with affinity-purified human Ro (SS-A). Nevertheless, except for a small percentage of Ro (SS-A) precipitin-positive sera, the frequency of antibody binding to the fusion protein was similar to the frequency of binding to the purified antigen in Western blots. Recombinant Ro (SS-A) antigen may therefore be valuable in the serologic evaluation of anti-Ro (SS-A) autoantibodies.

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Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

January 1990

Volume

33

Issue

1

Start / End Page

102 / 106

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Ribonucleoproteins
  • Rheumatic Diseases
  • Recombinant Fusion Proteins
  • RNA, Small Cytoplasmic
  • Molecular Weight
  • Immune Sera
  • Humans
  • Epitopes
  • Blotting, Western
 

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James, J. A., Dickey, W. D., Fujisaku, A., O’Brien, C. A., Deutscher, S. L., Keene, J. D., & Harley, J. B. (1990). Antigenicity of a recombinant Ro (SS-A) fusion protein. Arthritis Rheum, 33(1), 102–106. https://doi.org/10.1002/art.1780330114
James, J. A., W. D. Dickey, A. Fujisaku, C. A. O’Brien, S. L. Deutscher, J. D. Keene, and J. B. Harley. “Antigenicity of a recombinant Ro (SS-A) fusion protein.Arthritis Rheum 33, no. 1 (January 1990): 102–6. https://doi.org/10.1002/art.1780330114.
James JA, Dickey WD, Fujisaku A, O’Brien CA, Deutscher SL, Keene JD, et al. Antigenicity of a recombinant Ro (SS-A) fusion protein. Arthritis Rheum. 1990 Jan;33(1):102–6.
James, J. A., et al. “Antigenicity of a recombinant Ro (SS-A) fusion protein.Arthritis Rheum, vol. 33, no. 1, Jan. 1990, pp. 102–06. Pubmed, doi:10.1002/art.1780330114.
James JA, Dickey WD, Fujisaku A, O’Brien CA, Deutscher SL, Keene JD, Harley JB. Antigenicity of a recombinant Ro (SS-A) fusion protein. Arthritis Rheum. 1990 Jan;33(1):102–106.
Journal cover image

Published In

Arthritis Rheum

DOI

ISSN

0004-3591

Publication Date

January 1990

Volume

33

Issue

1

Start / End Page

102 / 106

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Ribonucleoproteins
  • Rheumatic Diseases
  • Recombinant Fusion Proteins
  • RNA, Small Cytoplasmic
  • Molecular Weight
  • Immune Sera
  • Humans
  • Epitopes
  • Blotting, Western