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Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines.

Publication ,  Journal Article
Negri, DRM; Rossi, A; Blasi, M; Michelini, Z; Leone, P; Chiantore, MV; Baroncelli, S; Perretta, G; Cimarelli, A; Klotman, ME; Cara, A
Published in: Retrovirology
August 22, 2012

BACKGROUND: Integrase defective lentiviral vectors (IDLV) represent a promising delivery system for immunization purposes. Human dendritic cells (DC) are the main cell types mediating the immune response and are readily transduced by IDLV, allowing effective triggering of in vitro expansion of antigen-specific primed CD8+ T cells. However, IDLV expression in transduced DC is at lower levels than those of the integrase (IN) competent counterpart, thus requiring further improvement of IDLV for future use in the clinic. RESULTS: In this paper we show that the addition of simian immunodeficiency (SIV)-Vpx protein in the vector preparation greatly improves transduction of human and simian DC, but not of murine DC, thus increasing the ability of transduced DC to act as functional antigen presenting cells, in the absence of integrated vector sequences. Importantly, the presence of SIV-Vpx allows for using lower dose of input IDLV during in vitro transduction, thus further improving the IDLV safety profile. CONCLUSIONS: These results have significant implications for the development of IDLV-based vaccines.

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Published In

Retrovirology

DOI

EISSN

1742-4690

Publication Date

August 22, 2012

Volume

9

Start / End Page

69

Location

England

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Viral Regulatory and Accessory Proteins
  • Transduction, Genetic
  • Mice, Inbred BALB C
  • Mice
  • Macaca mulatta
  • Macaca fascicularis
  • Lentivirus
  • Humans
 

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Negri, D. R. M., Rossi, A., Blasi, M., Michelini, Z., Leone, P., Chiantore, M. V., … Cara, A. (2012). Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines. Retrovirology, 9, 69. https://doi.org/10.1186/1742-4690-9-69
Negri, Donatella R. M., Alessandra Rossi, Maria Blasi, Zuleika Michelini, Pasqualina Leone, Maria Vincenza Chiantore, Silvia Baroncelli, et al. “Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines.Retrovirology 9 (August 22, 2012): 69. https://doi.org/10.1186/1742-4690-9-69.
Negri DRM, Rossi A, Blasi M, Michelini Z, Leone P, Chiantore MV, et al. Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines. Retrovirology. 2012 Aug 22;9:69.
Negri, Donatella R. M., et al. “Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines.Retrovirology, vol. 9, Aug. 2012, p. 69. Pubmed, doi:10.1186/1742-4690-9-69.
Negri DRM, Rossi A, Blasi M, Michelini Z, Leone P, Chiantore MV, Baroncelli S, Perretta G, Cimarelli A, Klotman ME, Cara A. Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines. Retrovirology. 2012 Aug 22;9:69.
Journal cover image

Published In

Retrovirology

DOI

EISSN

1742-4690

Publication Date

August 22, 2012

Volume

9

Start / End Page

69

Location

England

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Viral Regulatory and Accessory Proteins
  • Transduction, Genetic
  • Mice, Inbred BALB C
  • Mice
  • Macaca mulatta
  • Macaca fascicularis
  • Lentivirus
  • Humans