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Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice.

Publication ,  Journal Article
Negri, DRM; Michelini, Z; Baroncelli, S; Spada, M; Vendetti, S; Bona, R; Leone, P; Klotman, ME; Cara, A
Published in: J Biomed Biotechnol
2010

CD8+ T cells are an essential component of an effective host immune response to tumors and viral infections. Genetic immunization is particularly suitable for inducing CTL responses, because the encoded proteins enter the MHC class I processing pathway through either transgene expression or cross-presentation. In order to compare the efficiency and persistence of immune response induced by genetic vaccines, BALB/c mice were immunized either twice intramuscularly with DNA plasmid expressing a codon-optimized HIV-1 gp120 Envelope sequence together with murine GM-CSF sequence or with a single immunization using an integrase defective lentiviral vector (IDLV) expressing the same proteins. Results strongly indicated that the schedule based on IDLV vaccine was more efficient in inducing specific immune response, as evaluated three months after the last immunization by IFNgamma ELISPOT in both splenocytes and bone marrow- (BM-) derived cells, chromium release assay in splenocytes, and antibody detection in sera. In addition, IDLV immunization induced high frequency of polyfunctional CD8+ T cells able to simultaneously produce IFNgamma, TNFalpha, and IL2.

Duke Scholars

Published In

J Biomed Biotechnol

DOI

EISSN

1110-7251

Publication Date

2010

Volume

2010

Start / End Page

534501

Location

United States

Related Subject Headings

  • 10 Technology
  • 08 Information and Computing Sciences
  • 06 Biological Sciences
 

Citation

APA
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ICMJE
MLA
NLM
Negri, D. R. M., Michelini, Z., Baroncelli, S., Spada, M., Vendetti, S., Bona, R., … Cara, A. (2010). Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice. J Biomed Biotechnol, 2010, 534501. https://doi.org/10.1155/2010/534501
Negri, Donatella R. M., Zuleika Michelini, Silvia Baroncelli, Massimo Spada, Silvia Vendetti, Roberta Bona, Pasqualina Leone, Mary E. Klotman, and Andrea Cara. “Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice.J Biomed Biotechnol 2010 (2010): 534501. https://doi.org/10.1155/2010/534501.
Negri DRM, Michelini Z, Baroncelli S, Spada M, Vendetti S, Bona R, et al. Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice. J Biomed Biotechnol. 2010;2010:534501.
Negri, Donatella R. M., et al. “Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice.J Biomed Biotechnol, vol. 2010, 2010, p. 534501. Pubmed, doi:10.1155/2010/534501.
Negri DRM, Michelini Z, Baroncelli S, Spada M, Vendetti S, Bona R, Leone P, Klotman ME, Cara A. Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice. J Biomed Biotechnol. 2010;2010:534501.

Published In

J Biomed Biotechnol

DOI

EISSN

1110-7251

Publication Date

2010

Volume

2010

Start / End Page

534501

Location

United States

Related Subject Headings

  • 10 Technology
  • 08 Information and Computing Sciences
  • 06 Biological Sciences