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Replication and compartmentalization of HIV-1 in kidney epithelium of patients with HIV-associated nephropathy.

Publication ,  Journal Article
Marras, D; Bruggeman, LA; Gao, F; Tanji, N; Mansukhani, MM; Cara, A; Ross, MD; Gusella, GL; Benson, G; D'Agati, VD; Hahn, BH; Klotman, ME; Klotman, PE
Published in: Nat Med
May 2002

HIV-associated nephropathy is a clinicopathologic entity that includes proteinuria, focal segmental glomerulosclerosis often of the collapsing variant, and microcystic tubulointerstitial disease. Increasing evidence supports a role for HIV-1 infection of renal epithelium in the pathogenesis of HIV-associated nephropathy. Using in situ hybridization, we previously demonstrated HIV-1 gag and nef mRNA in renal epithelial cells of patients with HIV-associated nephropathy. Here, to investigate whether renal epithelial cells were productively infected by HIV-1, we examined renal tissue for the presence of HIV-1 DNA and mRNA by in situ hybridization and PCR, and we molecularly characterized the HIV-1 quasispecies in the renal compartment. Infected renal epithelial cells were removed by laser-capture microdissection from biopsies of two patients, DNA was extracted, and HIV-1 V3-loop or gp120-envelope sequences were amplified from individually dissected cells by nested PCR. Phylogenetic analysis of kidney-derived sequences as well as corresponding sequences from peripheral blood mononuclear cells of the same patients revealed evidence of tissue-specific viral evolution. In phylogenetic trees constructed from V3 and gp120 sequences, kidney-derived sequences formed tissue-specific subclusters within the radiation of blood mononuclear cell-derived viral sequences from both patients. These data, along with the detection of HIV-1-specific proviral DNA and mRNA in tubular epithelium cells, argue strongly for localized replication of HIV-1 in the kidney and the existence of a renal viral reservoir.

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Published In

Nat Med

DOI

ISSN

1078-8956

Publication Date

May 2002

Volume

8

Issue

5

Start / End Page

522 / 526

Location

United States

Related Subject Headings

  • Virus Replication
  • Urothelium
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Kidney Diseases
  • Kidney
  • Immunology
  • Humans
  • HIV-1
  • HIV Infections
 

Citation

APA
Chicago
ICMJE
MLA
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Marras, D., Bruggeman, L. A., Gao, F., Tanji, N., Mansukhani, M. M., Cara, A., … Klotman, P. E. (2002). Replication and compartmentalization of HIV-1 in kidney epithelium of patients with HIV-associated nephropathy. Nat Med, 8(5), 522–526. https://doi.org/10.1038/nm0502-522
Marras, Daniele, Leslie A. Bruggeman, Feng Gao, Nozomu Tanji, Mahesh M. Mansukhani, Andrea Cara, Michael D. Ross, et al. “Replication and compartmentalization of HIV-1 in kidney epithelium of patients with HIV-associated nephropathy.Nat Med 8, no. 5 (May 2002): 522–26. https://doi.org/10.1038/nm0502-522.
Marras D, Bruggeman LA, Gao F, Tanji N, Mansukhani MM, Cara A, et al. Replication and compartmentalization of HIV-1 in kidney epithelium of patients with HIV-associated nephropathy. Nat Med. 2002 May;8(5):522–6.
Marras, Daniele, et al. “Replication and compartmentalization of HIV-1 in kidney epithelium of patients with HIV-associated nephropathy.Nat Med, vol. 8, no. 5, May 2002, pp. 522–26. Pubmed, doi:10.1038/nm0502-522.
Marras D, Bruggeman LA, Gao F, Tanji N, Mansukhani MM, Cara A, Ross MD, Gusella GL, Benson G, D’Agati VD, Hahn BH, Klotman ME, Klotman PE. Replication and compartmentalization of HIV-1 in kidney epithelium of patients with HIV-associated nephropathy. Nat Med. 2002 May;8(5):522–526.

Published In

Nat Med

DOI

ISSN

1078-8956

Publication Date

May 2002

Volume

8

Issue

5

Start / End Page

522 / 526

Location

United States

Related Subject Headings

  • Virus Replication
  • Urothelium
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Kidney Diseases
  • Kidney
  • Immunology
  • Humans
  • HIV-1
  • HIV Infections