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Systemic production of human granulocyte colony-stimulating factor in nonhuman primates by transplantation of genetically modified myoblasts.

Publication ,  Journal Article
Moisset, PA; Bonham, L; Skuk, D; Koeberl, D; Brussee, V; Goulet, M; Roy, B; Asselin, I; Miller, AD; Tremblay, JP
Published in: Hum Gene Ther
June 10, 2000

Clinical use of human granulocyte-colony stimulating factor (hG-CSF) to treat various diseases involving neutropenia has been previously shown to (1) successfully increase circulating neutrophils, (2) reduce condition-related infections, and (3) cause few side effects in patients. To alleviate the symptoms of neutropenia, the patient must receive frequent injections of recombinant hG-CSF. Permanent ways to deliver stable levels of the molecule to the patient are being investigated. Among them, the transplantation of hG-CSF-secreting cells has been proposed and performed successfully in rodents, using fibroblast cell lines and primary muscle cells. We thus investigated whether similar results could be obtained by intramuscular myoblast transplantation in a large animal model. When 1-3 x 10(8) myoblasts were injected into three Macaca mulatta, hG-CSF was detected at high levels (300-900 pg/ml), which in turn led to a four- to fivefold increase in circulating neutrophils. However, both the concentrations of hG-CSF and neutrophil levels were found to decrease over time. Nonetheless, neutrophils were found at higher levels from the fourth week until the end the experiment (up to 29 weeks) in G-CSF monkeys compared with control animals. These results show that transplantation of hG-CSF-secreting myoblasts may indeed be a therapeutic option for the treatment of neutropenic patients.

Duke Scholars

Published In

Hum Gene Ther

DOI

ISSN

1043-0342

Publication Date

June 10, 2000

Volume

11

Issue

9

Start / End Page

1277 / 1288

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Time Factors
  • Recombinant Proteins
  • Neutrophils
  • Muscle, Skeletal
  • Mice, SCID
  • Mice, Inbred BALB C
  • Mice
  • Macaca mulatta
  • Injections, Intramuscular
 

Citation

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Moisset, P. A., Bonham, L., Skuk, D., Koeberl, D., Brussee, V., Goulet, M., … Tremblay, J. P. (2000). Systemic production of human granulocyte colony-stimulating factor in nonhuman primates by transplantation of genetically modified myoblasts. Hum Gene Ther, 11(9), 1277–1288. https://doi.org/10.1089/10430340050032384
Moisset, P. A., L. Bonham, D. Skuk, D. Koeberl, V. Brussee, M. Goulet, B. Roy, I. Asselin, A. D. Miller, and J. P. Tremblay. “Systemic production of human granulocyte colony-stimulating factor in nonhuman primates by transplantation of genetically modified myoblasts.Hum Gene Ther 11, no. 9 (June 10, 2000): 1277–88. https://doi.org/10.1089/10430340050032384.
Moisset PA, Bonham L, Skuk D, Koeberl D, Brussee V, Goulet M, et al. Systemic production of human granulocyte colony-stimulating factor in nonhuman primates by transplantation of genetically modified myoblasts. Hum Gene Ther. 2000 Jun 10;11(9):1277–88.
Moisset, P. A., et al. “Systemic production of human granulocyte colony-stimulating factor in nonhuman primates by transplantation of genetically modified myoblasts.Hum Gene Ther, vol. 11, no. 9, June 2000, pp. 1277–88. Pubmed, doi:10.1089/10430340050032384.
Moisset PA, Bonham L, Skuk D, Koeberl D, Brussee V, Goulet M, Roy B, Asselin I, Miller AD, Tremblay JP. Systemic production of human granulocyte colony-stimulating factor in nonhuman primates by transplantation of genetically modified myoblasts. Hum Gene Ther. 2000 Jun 10;11(9):1277–1288.
Journal cover image

Published In

Hum Gene Ther

DOI

ISSN

1043-0342

Publication Date

June 10, 2000

Volume

11

Issue

9

Start / End Page

1277 / 1288

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Time Factors
  • Recombinant Proteins
  • Neutrophils
  • Muscle, Skeletal
  • Mice, SCID
  • Mice, Inbred BALB C
  • Mice
  • Macaca mulatta
  • Injections, Intramuscular