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Direct ribosomal binding by a cellular inhibitor of translation.

Publication ,  Journal Article
Colón-Ramos, DA; Shenvi, CL; Weitzel, DH; Gan, EC; Matts, R; Cate, J; Kornbluth, S
Published in: Nature structural & molecular biology
February 2006

During apoptosis and under conditions of cellular stress, several signaling pathways promote inhibition of cap-dependent translation while allowing continued translation of specific messenger RNAs encoding regulatory and stress-response proteins. We report here that the apoptotic regulator Reaper inhibits protein synthesis by binding directly to the 40S ribosomal subunit. This interaction does not affect either ribosomal association of initiation factors or formation of 43S or 48S complexes. Rather, it interferes with late initiation events upstream of 60S subunit joining, apparently modulating start-codon recognition during scanning. CrPV IRES-driven translation, involving direct ribosomal recruitment to the start site, is relatively insensitive to Reaper. Thus, Reaper is the first known cellular ribosomal binding factor with the potential to allow selective translation of mRNAs initiating at alternative start codons or from certain IRES elements. This function of Reaper may modulate gene expression programs to affect cell fate.

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Published In

Nature structural & molecular biology

DOI

EISSN

1545-9985

ISSN

1545-9993

Publication Date

February 2006

Volume

13

Issue

2

Start / End Page

103 / 111

Related Subject Headings

  • Ribosomes
  • Rabbits
  • RNA, Messenger
  • Protein Biosynthesis
  • Protein Binding
  • Phosphorylation
  • Drosophila melanogaster
  • Drosophila Proteins
  • Developmental Biology
  • DNA Repair
 

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Colón-Ramos, D. A., Shenvi, C. L., Weitzel, D. H., Gan, E. C., Matts, R., Cate, J., & Kornbluth, S. (2006). Direct ribosomal binding by a cellular inhibitor of translation. Nature Structural & Molecular Biology, 13(2), 103–111. https://doi.org/10.1038/nsmb1052
Colón-Ramos, Daniel A., Christina L. Shenvi, Douglas H. Weitzel, Eugene C. Gan, Robert Matts, Jamie Cate, and Sally Kornbluth. “Direct ribosomal binding by a cellular inhibitor of translation.Nature Structural & Molecular Biology 13, no. 2 (February 2006): 103–11. https://doi.org/10.1038/nsmb1052.
Colón-Ramos DA, Shenvi CL, Weitzel DH, Gan EC, Matts R, Cate J, et al. Direct ribosomal binding by a cellular inhibitor of translation. Nature structural & molecular biology. 2006 Feb;13(2):103–11.
Colón-Ramos, Daniel A., et al. “Direct ribosomal binding by a cellular inhibitor of translation.Nature Structural & Molecular Biology, vol. 13, no. 2, Feb. 2006, pp. 103–11. Epmc, doi:10.1038/nsmb1052.
Colón-Ramos DA, Shenvi CL, Weitzel DH, Gan EC, Matts R, Cate J, Kornbluth S. Direct ribosomal binding by a cellular inhibitor of translation. Nature structural & molecular biology. 2006 Feb;13(2):103–111.

Published In

Nature structural & molecular biology

DOI

EISSN

1545-9985

ISSN

1545-9993

Publication Date

February 2006

Volume

13

Issue

2

Start / End Page

103 / 111

Related Subject Headings

  • Ribosomes
  • Rabbits
  • RNA, Messenger
  • Protein Biosynthesis
  • Protein Binding
  • Phosphorylation
  • Drosophila melanogaster
  • Drosophila Proteins
  • Developmental Biology
  • DNA Repair