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Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase.

Publication ,  Journal Article
Casaletto, JB; Nutt, LK; Wu, Q; Moore, JD; Etkin, LD; Jackson, PK; Hunt, T; Kornbluth, S
Published in: The Journal of cell biology
April 2005

Degradation of specific protein substrates by the anaphase-promoting complex/cyclosome (APC) is critical for mitotic exit. We have identified the protein Xenopus nuclear factor 7 (Xnf7) as a novel APC inhibitor able to regulate the timing of exit from mitosis. Immunodepletion of Xnf7 from Xenopus laevis egg extracts accelerated the degradation of APC substrates cyclin B1, cyclin B2, and securin upon release from cytostatic factor arrest, whereas excess Xnf7 inhibited APC activity. Interestingly, Xnf7 exhibited intrinsic ubiquitin ligase activity, and this activity was required for APC inhibition. Unlike other reported APC inhibitors, Xnf7 did not associate with Cdc20, but rather bound directly to core subunits of the APC. Furthermore, Xnf7 was required for spindle assembly checkpoint function in egg extracts. These data suggest that Xnf7 is an APC inhibitor able to link spindle status to the APC through direct association with APC core components.

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Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

April 2005

Volume

169

Issue

1

Start / End Page

61 / 71

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Ubiquitin-Protein Ligase Complexes
  • Spindle Apparatus
  • Recombinant Proteins
  • Protein Binding
  • Phosphoproteins
  • Oocytes
  • Nuclear Proteins
  • Mitosis
 

Citation

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Casaletto, J. B., Nutt, L. K., Wu, Q., Moore, J. D., Etkin, L. D., Jackson, P. K., … Kornbluth, S. (2005). Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase. The Journal of Cell Biology, 169(1), 61–71. https://doi.org/10.1083/jcb.200411056
Casaletto, Jessica B., Leta K. Nutt, Qiju Wu, Jonathan D. Moore, Laurence D. Etkin, Peter K. Jackson, Tim Hunt, and Sally Kornbluth. “Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase.The Journal of Cell Biology 169, no. 1 (April 2005): 61–71. https://doi.org/10.1083/jcb.200411056.
Casaletto JB, Nutt LK, Wu Q, Moore JD, Etkin LD, Jackson PK, et al. Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase. The Journal of cell biology. 2005 Apr;169(1):61–71.
Casaletto, Jessica B., et al. “Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase.The Journal of Cell Biology, vol. 169, no. 1, Apr. 2005, pp. 61–71. Epmc, doi:10.1083/jcb.200411056.
Casaletto JB, Nutt LK, Wu Q, Moore JD, Etkin LD, Jackson PK, Hunt T, Kornbluth S. Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase. The Journal of cell biology. 2005 Apr;169(1):61–71.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

April 2005

Volume

169

Issue

1

Start / End Page

61 / 71

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Ubiquitin-Protein Ligase Complexes
  • Spindle Apparatus
  • Recombinant Proteins
  • Protein Binding
  • Phosphoproteins
  • Oocytes
  • Nuclear Proteins
  • Mitosis