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DNA replication checkpoint control of Wee1 stability by vertebrate Hsl7.

Publication ,  Journal Article
Yamada, A; Duffy, B; Perry, JA; Kornbluth, S
Published in: The Journal of cell biology
December 2004

G2/M checkpoints prevent mitotic entry upon DNA damage or replication inhibition by targeting the Cdc2 regulators Cdc25 and Wee1. Although Wee1 protein stability is regulated by DNA-responsive checkpoints, the vertebrate pathways controlling Wee1 degradation have not been elucidated. In budding yeast, stability of the Wee1 homologue, Swe1, is controlled by a regulatory module consisting of the proteins Hsl1 and Hsl7 (histone synthetic lethal 1 and 7), which are targeted by the morphogenesis checkpoint to prevent Swe1 degradation when budding is inhibited. We report here the identification of Xenopus Hsl7 as a positive regulator of mitosis that is controlled, instead, by an entirely distinct checkpoint, the DNA replication checkpoint. Although inhibiting Hsl7 delayed mitosis, Hsl7 overexpression overrode the replication checkpoint, accelerating Wee1 destruction. Replication checkpoint activation disrupted Hsl7-Wee1 interactions, but binding was restored by active polo-like kinase. These data establish Hsl7 as a component of the replication checkpoint and reveal that similar cell cycle control modules can be co-opted for use by distinct checkpoints in different organisms.

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Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

December 2004

Volume

167

Issue

5

Start / End Page

841 / 849

Related Subject Headings

  • Xenopus laevis
  • Xenopus Proteins
  • Vertebrates
  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Schizosaccharomyces pombe Proteins
  • Saccharomyces cerevisiae Proteins
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
 

Citation

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Yamada, A., Duffy, B., Perry, J. A., & Kornbluth, S. (2004). DNA replication checkpoint control of Wee1 stability by vertebrate Hsl7. The Journal of Cell Biology, 167(5), 841–849. https://doi.org/10.1083/jcb.200406048
Yamada, Ayumi, Brad Duffy, Jennifer A. Perry, and Sally Kornbluth. “DNA replication checkpoint control of Wee1 stability by vertebrate Hsl7.The Journal of Cell Biology 167, no. 5 (December 2004): 841–49. https://doi.org/10.1083/jcb.200406048.
Yamada A, Duffy B, Perry JA, Kornbluth S. DNA replication checkpoint control of Wee1 stability by vertebrate Hsl7. The Journal of cell biology. 2004 Dec;167(5):841–9.
Yamada, Ayumi, et al. “DNA replication checkpoint control of Wee1 stability by vertebrate Hsl7.The Journal of Cell Biology, vol. 167, no. 5, Dec. 2004, pp. 841–49. Epmc, doi:10.1083/jcb.200406048.
Yamada A, Duffy B, Perry JA, Kornbluth S. DNA replication checkpoint control of Wee1 stability by vertebrate Hsl7. The Journal of cell biology. 2004 Dec;167(5):841–849.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

December 2004

Volume

167

Issue

5

Start / End Page

841 / 849

Related Subject Headings

  • Xenopus laevis
  • Xenopus Proteins
  • Vertebrates
  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Schizosaccharomyces pombe Proteins
  • Saccharomyces cerevisiae Proteins
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases