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Post-cytochrome C protection from apoptosis conferred by a MAPK pathway in Xenopus egg extracts.

Publication ,  Journal Article
Tashker, JS; Olson, M; Kornbluth, S
Published in: Molecular biology of the cell
February 2002

In response to many different apoptotic stimuli, cytochrome c is released from the intermembrane space of the mitochondria into the cytoplasm, where it serves as a cofactor in the activation of procaspase 9. Inhibition of this process can occur either by preventing cytochrome c release or by blocking caspase activation or activity. Experiments involving in vitro reconstitution of apoptosis in cell-free extracts of Xenopus laevis eggs have suggested that extracts arrested in interphase are susceptible to an endogenous apoptotic program leading to caspase activation, whereas extracts arrested in meiotic metaphase are not. We report here that Mos/MEK/MAPK pathways active in M phase-arrested eggs are responsible for rendering them refractory to apoptosis. Interestingly, M phase-arrested extracts are competent to release cytochrome c, yet still do not activate caspases. Concomitantly, we have also demonstrated that recombinant Mos, MEK, and ERK are sufficient to block cytochrome c-dependent caspase activation in purified Xenopus cytosol, which lacks both transcription and translation. These data indicate that the MAP kinase pathway can target and inhibit post-cytochrome c release apoptotic events in the absence of new mRNA/protein synthesis and that this biochemical pathway is responsible for the apoptotic inhibition observed in meiotic X. laevis egg extracts.

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Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

February 2002

Volume

13

Issue

2

Start / End Page

393 / 401

Related Subject Headings

  • Xenopus laevis
  • Signal Transduction
  • Ovum
  • Mitogen-Activated Protein Kinases
  • Mitochondria
  • Meiosis
  • Interphase
  • Female
  • Developmental Biology
  • Cytochrome c Group
 

Citation

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Tashker, J. S., Olson, M., & Kornbluth, S. (2002). Post-cytochrome C protection from apoptosis conferred by a MAPK pathway in Xenopus egg extracts. Molecular Biology of the Cell, 13(2), 393–401. https://doi.org/10.1091/mbc.01-06-0291
Tashker, Jessica S., Michael Olson, and Sally Kornbluth. “Post-cytochrome C protection from apoptosis conferred by a MAPK pathway in Xenopus egg extracts.Molecular Biology of the Cell 13, no. 2 (February 2002): 393–401. https://doi.org/10.1091/mbc.01-06-0291.
Tashker JS, Olson M, Kornbluth S. Post-cytochrome C protection from apoptosis conferred by a MAPK pathway in Xenopus egg extracts. Molecular biology of the cell. 2002 Feb;13(2):393–401.
Tashker, Jessica S., et al. “Post-cytochrome C protection from apoptosis conferred by a MAPK pathway in Xenopus egg extracts.Molecular Biology of the Cell, vol. 13, no. 2, Feb. 2002, pp. 393–401. Epmc, doi:10.1091/mbc.01-06-0291.
Tashker JS, Olson M, Kornbluth S. Post-cytochrome C protection from apoptosis conferred by a MAPK pathway in Xenopus egg extracts. Molecular biology of the cell. 2002 Feb;13(2):393–401.

Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

February 2002

Volume

13

Issue

2

Start / End Page

393 / 401

Related Subject Headings

  • Xenopus laevis
  • Signal Transduction
  • Ovum
  • Mitogen-Activated Protein Kinases
  • Mitochondria
  • Meiosis
  • Interphase
  • Female
  • Developmental Biology
  • Cytochrome c Group