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Analysis of apoptosis using Xenopus egg extracts.

Publication ,  Journal Article
Kornbluth, S; Evans, EK
Published in: Current protocols in cell biology
May 2001

In the presence of a subcellular fraction enriched for mitochondria, after prolonged incubation the Xenopus egg extract can mimic biochemical aspects of apoptosis such as caspase activation, and DNA fragmentation. This unit describes preparation of an apoptotic extract from a crude interphase extract and an extract fractionated into latent and execution phases. An apoptotic extract can also be reconstituted from a fractionated interphase extract and purified mitochondria. Protocols are also included for monitoring apoptotic progression in the extract either by following activation of apoptotic proteases (caspases) or by assessing translocation of cytochrome c from the mitochondria to the cytosol.

Duke Scholars

Published In

Current protocols in cell biology

DOI

EISSN

1934-2616

ISSN

1934-2500

Publication Date

May 2001

Volume

Chapter 11

Start / End Page

Unit / 11.12

Related Subject Headings

  • Xenopus
  • Oocytes
  • Interphase
  • Female
  • Cytological Techniques
  • Cell Fractionation
  • Cell Extracts
  • Apoptosis
  • Animals
  • 3101 Biochemistry and cell biology
 

Citation

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Kornbluth, S., & Evans, E. K. (2001). Analysis of apoptosis using Xenopus egg extracts. Current Protocols in Cell Biology, Chapter 11, Unit-11.12. https://doi.org/10.1002/0471143030.cb1112s09
Kornbluth, S., and E. K. Evans. “Analysis of apoptosis using Xenopus egg extracts.Current Protocols in Cell Biology Chapter 11 (May 2001): Unit-11.12. https://doi.org/10.1002/0471143030.cb1112s09.
Kornbluth S, Evans EK. Analysis of apoptosis using Xenopus egg extracts. Current protocols in cell biology. 2001 May;Chapter 11:Unit-11.12.
Kornbluth, S., and E. K. Evans. “Analysis of apoptosis using Xenopus egg extracts.Current Protocols in Cell Biology, vol. Chapter 11, May 2001, p. Unit-11.12. Epmc, doi:10.1002/0471143030.cb1112s09.
Kornbluth S, Evans EK. Analysis of apoptosis using Xenopus egg extracts. Current protocols in cell biology. 2001 May;Chapter 11:Unit-11.12.

Published In

Current protocols in cell biology

DOI

EISSN

1934-2616

ISSN

1934-2500

Publication Date

May 2001

Volume

Chapter 11

Start / End Page

Unit / 11.12

Related Subject Headings

  • Xenopus
  • Oocytes
  • Interphase
  • Female
  • Cytological Techniques
  • Cell Fractionation
  • Cell Extracts
  • Apoptosis
  • Animals
  • 3101 Biochemistry and cell biology