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Preparation and use of interphase Xenopus egg extracts.

Publication ,  Journal Article
Powers, M; Evans, EK; Yang, J; Kornbluth, S
Published in: Current protocols in cell biology
May 2001

In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.

Duke Scholars

Published In

Current protocols in cell biology

DOI

EISSN

1934-2616

ISSN

1934-2500

Publication Date

May 2001

Volume

Chapter 11

Start / End Page

Unit / 11.10

Related Subject Headings

  • Xenopus
  • Oocytes
  • Interphase
  • Female
  • DNA Replication
  • Cytological Techniques
  • Cell Extracts
  • Animals
  • Active Transport, Cell Nucleus
  • 3101 Biochemistry and cell biology
 

Citation

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Powers, M., Evans, E. K., Yang, J., & Kornbluth, S. (2001). Preparation and use of interphase Xenopus egg extracts. Current Protocols in Cell Biology, Chapter 11, Unit-11.10. https://doi.org/10.1002/0471143030.cb1110s09
Powers, M., E. K. Evans, J. Yang, and S. Kornbluth. “Preparation and use of interphase Xenopus egg extracts.Current Protocols in Cell Biology Chapter 11 (May 2001): Unit-11.10. https://doi.org/10.1002/0471143030.cb1110s09.
Powers M, Evans EK, Yang J, Kornbluth S. Preparation and use of interphase Xenopus egg extracts. Current protocols in cell biology. 2001 May;Chapter 11:Unit-11.10.
Powers, M., et al. “Preparation and use of interphase Xenopus egg extracts.Current Protocols in Cell Biology, vol. Chapter 11, May 2001, p. Unit-11.10. Epmc, doi:10.1002/0471143030.cb1110s09.
Powers M, Evans EK, Yang J, Kornbluth S. Preparation and use of interphase Xenopus egg extracts. Current protocols in cell biology. 2001 May;Chapter 11:Unit-11.10.

Published In

Current protocols in cell biology

DOI

EISSN

1934-2616

ISSN

1934-2500

Publication Date

May 2001

Volume

Chapter 11

Start / End Page

Unit / 11.10

Related Subject Headings

  • Xenopus
  • Oocytes
  • Interphase
  • Female
  • DNA Replication
  • Cytological Techniques
  • Cell Extracts
  • Animals
  • Active Transport, Cell Nucleus
  • 3101 Biochemistry and cell biology