Skip to main content
Journal cover image

A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch.

Publication ,  Journal Article
Kaye, FJ; Modi, S; Ivanovska, I; Koonin, EV; Thress, K; Kubo, A; Kornbluth, S; Rose, MD
Published in: FEBS letters
February 2000

We have isolated two human ubiquitin-like (UbL) proteins that bind to a short peptide within the ATPase domain of the Hsp70-like Stch protein. Chap1 is a duplicated homologue of the yeast Dsk2 gene that is required for transit through the G2/M phase of the cell cycle and expression of the human full-length cDNA restored viability and suppressed the G2/M arrest phenotype of dsk2Delta rad23Delta Saccharomyces cerevisiae mutants. Chap2 is a homologue for Xenopus scythe which is an essential component of reaper-induced apoptosis in egg extracts. While the N-terminal UbL domains were not essential for Stch binding, Chap1/Dsk2 contains a Sti1-like repeat sequence that is required for binding to Stch and is also conserved in the Hsp70 binding proteins, Hip and p60/Sti1/Hop. These findings extend the association between Hsp70 members and genes encoding UbL sequences and suggest a broader role for the Hsp70-like ATPase family in regulating cell cycle and cell death events.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

FEBS letters

DOI

EISSN

1873-3468

ISSN

0014-5793

Publication Date

February 2000

Volume

467

Issue

2-3

Start / End Page

348 / 355

Related Subject Headings

  • Ubiquitins
  • Sequence Alignment
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Molecular Sequence Data
  • Humans
  • HSP70 Heat-Shock Proteins
  • Fungal Proteins
  • Cell Cycle Proteins
  • Biochemistry & Molecular Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kaye, F. J., Modi, S., Ivanovska, I., Koonin, E. V., Thress, K., Kubo, A., … Rose, M. D. (2000). A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch. FEBS Letters, 467(2–3), 348–355. https://doi.org/10.1016/s0014-5793(00)01135-2
Kaye, F. J., S. Modi, I. Ivanovska, E. V. Koonin, K. Thress, A. Kubo, S. Kornbluth, and M. D. Rose. “A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch.FEBS Letters 467, no. 2–3 (February 2000): 348–55. https://doi.org/10.1016/s0014-5793(00)01135-2.
Kaye FJ, Modi S, Ivanovska I, Koonin EV, Thress K, Kubo A, et al. A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch. FEBS letters. 2000 Feb;467(2–3):348–55.
Kaye, F. J., et al. “A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch.FEBS Letters, vol. 467, no. 2–3, Feb. 2000, pp. 348–55. Epmc, doi:10.1016/s0014-5793(00)01135-2.
Kaye FJ, Modi S, Ivanovska I, Koonin EV, Thress K, Kubo A, Kornbluth S, Rose MD. A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch. FEBS letters. 2000 Feb;467(2–3):348–355.
Journal cover image

Published In

FEBS letters

DOI

EISSN

1873-3468

ISSN

0014-5793

Publication Date

February 2000

Volume

467

Issue

2-3

Start / End Page

348 / 355

Related Subject Headings

  • Ubiquitins
  • Sequence Alignment
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Molecular Sequence Data
  • Humans
  • HSP70 Heat-Shock Proteins
  • Fungal Proteins
  • Cell Cycle Proteins
  • Biochemistry & Molecular Biology