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Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1.

Publication ,  Journal Article
Moore, JD; Yang, J; Truant, R; Kornbluth, S
Published in: The Journal of cell biology
January 1999

Reversible phosphorylation of nuclear proteins is required for both DNA replication and entry into mitosis. Consequently, most cyclin-dependent kinase (Cdk)/cyclin complexes are localized to the nucleus when active. Although our understanding of nuclear transport processes has been greatly enhanced by the recent identification of nuclear targeting sequences and soluble nuclear import factors with which they interact, the mechanisms used to target Cdk/cyclin complexes to the nucleus remain obscure; this is in part because these proteins lack obvious nuclear localization sequences. To elucidate the molecular mechanisms responsible for Cdk/cyclin transport, we examined nuclear import of fluorescent Cdk2/cyclin E and Cdc2/cyclin B1 complexes in digitonin-permeabilized mammalian cells and also examined potential physical interactions between these Cdks, cyclins, and soluble import factors. We found that the nuclear import machinery recognizes these Cdk/cyclin complexes through direct interactions with the cyclin component. Surprisingly, cyclins E and B1 are imported into nuclei via distinct mechanisms. Cyclin E behaves like a classical basic nuclear localization sequence-containing protein, binding to the alpha adaptor subunit of the importin-alpha/beta heterodimer. In contrast, cyclin B1 is imported via a direct interaction with a site in the NH2 terminus of importin-beta that is distinct from that used to bind importin-alpha.

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Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

January 1999

Volume

144

Issue

2

Start / End Page

213 / 224

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Recombinant Fusion Proteins
  • Protein Serine-Threonine Kinases
  • Peptides
  • Nuclear Proteins
  • Karyopherins
  • Humans
  • Developmental Biology
  • Cyclin-Dependent Kinases
 

Citation

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Moore, J. D., Yang, J., Truant, R., & Kornbluth, S. (1999). Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1. The Journal of Cell Biology, 144(2), 213–224. https://doi.org/10.1083/jcb.144.2.213
Moore, J. D., J. Yang, R. Truant, and S. Kornbluth. “Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1.The Journal of Cell Biology 144, no. 2 (January 1999): 213–24. https://doi.org/10.1083/jcb.144.2.213.
Moore JD, Yang J, Truant R, Kornbluth S. Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1. The Journal of cell biology. 1999 Jan;144(2):213–24.
Moore, J. D., et al. “Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1.The Journal of Cell Biology, vol. 144, no. 2, Jan. 1999, pp. 213–24. Epmc, doi:10.1083/jcb.144.2.213.
Moore JD, Yang J, Truant R, Kornbluth S. Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1. The Journal of cell biology. 1999 Jan;144(2):213–224.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

January 1999

Volume

144

Issue

2

Start / End Page

213 / 224

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Recombinant Fusion Proteins
  • Protein Serine-Threonine Kinases
  • Peptides
  • Nuclear Proteins
  • Karyopherins
  • Humans
  • Developmental Biology
  • Cyclin-Dependent Kinases