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Scythe: a novel reaper-binding apoptotic regulator.

Publication ,  Journal Article
Thress, K; Henzel, W; Shillinglaw, W; Kornbluth, S
Published in: The EMBO journal
November 1998

Reaper is a central regulator of apoptosis in Drosophila melanogaster. With no obvious catalytic activity or homology to other known apoptotic regulators, reaper's mechanism of action has been obscure. We recently reported that recombinant Drosophila reaper protein induced rapid mitochondrial cytochrome c release, caspase activation and apoptotic nuclear fragmentation in extracts of Xenopus eggs. We now report the purification of a 150 kDa reaper-interacting protein from Xenopus egg extracts, which we have named Scythe. Scythe is highly conserved among vertebrates and contains a ubiquitin-like domain near its N-terminus. Immunodepletion of Scythe from extracts completely prevented reaper-induced apoptosis without affecting apoptosis triggered by activated caspases. Moreover, a truncated variant of Scythe lacking the N-terminal domain induced apoptosis even in the absence of reaper. These data suggest that Scythe is a novel apoptotic regulator that is an essential component in the pathway of reaper-induced apoptosis.

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Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

November 1998

Volume

17

Issue

21

Start / End Page

6135 / 6143

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Sequence Analysis, DNA
  • Recombinant Proteins
  • Protein Binding
  • Peptides
  • Peptide Hydrolases
  • Peptide Fragments
  • Oocytes
  • Molecular Sequence Data
 

Citation

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Thress, K., Henzel, W., Shillinglaw, W., & Kornbluth, S. (1998). Scythe: a novel reaper-binding apoptotic regulator. The EMBO Journal, 17(21), 6135–6143. https://doi.org/10.1093/emboj/17.21.6135
Thress, K., W. Henzel, W. Shillinglaw, and S. Kornbluth. “Scythe: a novel reaper-binding apoptotic regulator.The EMBO Journal 17, no. 21 (November 1998): 6135–43. https://doi.org/10.1093/emboj/17.21.6135.
Thress K, Henzel W, Shillinglaw W, Kornbluth S. Scythe: a novel reaper-binding apoptotic regulator. The EMBO journal. 1998 Nov;17(21):6135–43.
Thress, K., et al. “Scythe: a novel reaper-binding apoptotic regulator.The EMBO Journal, vol. 17, no. 21, Nov. 1998, pp. 6135–43. Epmc, doi:10.1093/emboj/17.21.6135.
Thress K, Henzel W, Shillinglaw W, Kornbluth S. Scythe: a novel reaper-binding apoptotic regulator. The EMBO journal. 1998 Nov;17(21):6135–6143.

Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

November 1998

Volume

17

Issue

21

Start / End Page

6135 / 6143

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Sequence Analysis, DNA
  • Recombinant Proteins
  • Protein Binding
  • Peptides
  • Peptide Hydrolases
  • Peptide Fragments
  • Oocytes
  • Molecular Sequence Data