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Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7.

Publication ,  Journal Article
Sterner, JM; Dew-Knight, S; Musahl, C; Kornbluth, S; Horowitz, JM
Published in: Molecular and cellular biology
May 1998

A yeast two-hybrid screen was employed to identify human proteins that specifically bind the amino-terminal 400 amino acids of the retinoblastoma (Rb) protein. Two independent cDNAs resulting from this screen were found to encode the carboxy-terminal 137 amino acids of MCM7, a member of a family of proteins that comprise replication licensing factor. Full-length Rb and MCM7 form protein complexes in vitro, and the amino termini of two Rb-related proteins, p107 and p130, also bind MCM7. Protein complexes between Rb and MCM7 were also detected in anti-Rb immunoprecipitates prepared from human cells. The amino-termini of Rb and p130 strongly inhibited DNA replication in an MCM7-dependent fashion in a Xenopus in vitro DNA replication assay system. These data provide the first evidence that Rb and Rb-related proteins can directly regulate DNA replication and that components of licensing factor are targets of the products of tumor suppressor genes.

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Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

May 1998

Volume

18

Issue

5

Start / End Page

2748 / 2757

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Spermatozoa
  • Retinoblastoma-Like Protein p130
  • Retinoblastoma-Like Protein p107
  • Retinoblastoma Protein
  • Recombinant Proteins
  • Proteins
  • Protein Binding
  • Phosphoproteins
 

Citation

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Sterner, J. M., Dew-Knight, S., Musahl, C., Kornbluth, S., & Horowitz, J. M. (1998). Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. Molecular and Cellular Biology, 18(5), 2748–2757. https://doi.org/10.1128/mcb.18.5.2748
Sterner, J. M., S. Dew-Knight, C. Musahl, S. Kornbluth, and J. M. Horowitz. “Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7.Molecular and Cellular Biology 18, no. 5 (May 1998): 2748–57. https://doi.org/10.1128/mcb.18.5.2748.
Sterner JM, Dew-Knight S, Musahl C, Kornbluth S, Horowitz JM. Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. Molecular and cellular biology. 1998 May;18(5):2748–57.
Sterner, J. M., et al. “Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7.Molecular and Cellular Biology, vol. 18, no. 5, May 1998, pp. 2748–57. Epmc, doi:10.1128/mcb.18.5.2748.
Sterner JM, Dew-Knight S, Musahl C, Kornbluth S, Horowitz JM. Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. Molecular and cellular biology. 1998 May;18(5):2748–2757.

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

May 1998

Volume

18

Issue

5

Start / End Page

2748 / 2757

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Spermatozoa
  • Retinoblastoma-Like Protein p130
  • Retinoblastoma-Like Protein p107
  • Retinoblastoma Protein
  • Recombinant Proteins
  • Proteins
  • Protein Binding
  • Phosphoproteins