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Regulation of apoptotic signaling pathways in cell-free extracts

Publication ,  Journal Article
Kornbluth, S
Published in: FASEB Journal
December 1, 1998

ApoptOBis is a program of cellular suicide which rids multicellular otanisms of damaged or extraneous cells. In order to elucidate the cellular signaling pathways which contribute to apoptotic cell death, we are utilizing an extract of Xenopus eggs which can recapitulate the apoptotic process in vitro. Using these extracts, we have found that the SH2/SH3-containlng adaptor protein, crk, is required for in vitro apoptosls. Moreover, proteins interacting with both the SH2 and SH3 domains are critical for transmitting the apoptotic signal. We are currently purifying proteins interacting with these domains. We have also found that the reaper protein of Drosophila Melanogaster, critical for apoptosis in that organism, can induce rapid apoptosis in extracts. Using reaper as a bait in affinity Chromatography experiments, we have purified a reaper-Interacting protein from egg extracts. Characterization of this protein will be discussed.

Duke Scholars

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

December 1, 1998

Volume

12

Issue

8

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology
 

Citation

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MLA
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Kornbluth, S. (1998). Regulation of apoptotic signaling pathways in cell-free extracts. FASEB Journal, 12(8).
Kornbluth, S. “Regulation of apoptotic signaling pathways in cell-free extracts.” FASEB Journal 12, no. 8 (December 1, 1998).
Kornbluth S. Regulation of apoptotic signaling pathways in cell-free extracts. FASEB Journal. 1998 Dec 1;12(8).
Kornbluth, S. “Regulation of apoptotic signaling pathways in cell-free extracts.” FASEB Journal, vol. 12, no. 8, Dec. 1998.
Kornbluth S. Regulation of apoptotic signaling pathways in cell-free extracts. FASEB Journal. 1998 Dec 1;12(8).

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

December 1, 1998

Volume

12

Issue

8

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology