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Crk is required for apoptosis in Xenopus egg extracts.

Publication ,  Journal Article
Evans, EK; Lu, W; Strum, SL; Mayer, BJ; Kornbluth, S
Published in: The EMBO journal
January 1997

Apoptosis is essential for the development and homeostasis of multicellular organisms. Recently, a cell-free extract prepared from Xenopus eggs was shown to recapitulate intracellular apoptotic pathways in vitro. While many stimuli have been shown to trigger apoptosis in a variety of cell types, the intracellular signaling pathways involved in apoptosis remain largely unknown. Here we show that addition of a recombinant protein containing the phosphotyrosine binding (SH2) domain from the adaptor protein crk, but not those derived from a panel of other signaling proteins, can prevent apoptosis in the Xenopus egg extract system. Furthermore, immunodepletion of endogenous crk protein from the egg extracts, or addition of anti-crk antisera to these extracts, prevents apoptosis. The ability to undergo apoptosis can be restored to these extracts by addition of recombinant crk protein. These results directly demonstrate that crk participates in apoptotic signaling.

Duke Scholars

Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

January 1997

Volume

16

Issue

2

Start / End Page

230 / 241

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Sequence Alignment
  • Proto-Oncogene Proteins c-crk
  • Proto-Oncogene Proteins
  • Phosphotyrosine
  • Oocytes
  • Molecular Sequence Data
  • Humans
  • Fusion Proteins, bcr-abl
 

Citation

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Evans, E. K., Lu, W., Strum, S. L., Mayer, B. J., & Kornbluth, S. (1997). Crk is required for apoptosis in Xenopus egg extracts. The EMBO Journal, 16(2), 230–241. https://doi.org/10.1093/emboj/16.2.230
Evans, E. K., W. Lu, S. L. Strum, B. J. Mayer, and S. Kornbluth. “Crk is required for apoptosis in Xenopus egg extracts.The EMBO Journal 16, no. 2 (January 1997): 230–41. https://doi.org/10.1093/emboj/16.2.230.
Evans EK, Lu W, Strum SL, Mayer BJ, Kornbluth S. Crk is required for apoptosis in Xenopus egg extracts. The EMBO journal. 1997 Jan;16(2):230–41.
Evans, E. K., et al. “Crk is required for apoptosis in Xenopus egg extracts.The EMBO Journal, vol. 16, no. 2, Jan. 1997, pp. 230–41. Epmc, doi:10.1093/emboj/16.2.230.
Evans EK, Lu W, Strum SL, Mayer BJ, Kornbluth S. Crk is required for apoptosis in Xenopus egg extracts. The EMBO journal. 1997 Jan;16(2):230–241.

Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

January 1997

Volume

16

Issue

2

Start / End Page

230 / 241

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Sequence Alignment
  • Proto-Oncogene Proteins c-crk
  • Proto-Oncogene Proteins
  • Phosphotyrosine
  • Oocytes
  • Molecular Sequence Data
  • Humans
  • Fusion Proteins, bcr-abl